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What is the Average, Standard Deviation (SD), Coefficient of Variation (CV) and % cell density group for your data? Cell Density AVG SD CV %CV A B C D E 100,000 50,000 25,000 12,500 6,250 F 3,125 G H 1,500 0

What is the Average, Standard Deviation (SD), Coefficient of Variation (CV) and % cell density group for your data? Cell Density AVG SD CV %CV A B C D E 100,000 50,000 25,000 12,500 6,250 F 3,125 G H 1,500 0

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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First page is reference for number 4-7 

PART A: INOCULATION ANALYSIS [Reference the Analysis instructions of the SRB Inoculation assay protocol.] 1. What is the Average, Standard Deviation (SD), Coefficient of Variation (CV) and %CV for each cell density group for your data? Cell Density AVG SD CV %CV A B C D E 100,000 50,000 25,000 12,500 6,250 2. What is the equation of your regression line? 3. What is the r² value for your data? F G H 3,125 1,500 0 DIAM 10 MURSY 1010 UDW JN IDW 4. Using the values above, determine the optimal cell number to plate for cytotoxicity assay. This should be done based on the combination of two factors: 1. Low CV value 2. Linearity (r2 value)
Transcribed Image Text:PART A: INOCULATION ANALYSIS [Reference the Analysis instructions of the SRB Inoculation assay protocol.] 1. What is the Average, Standard Deviation (SD), Coefficient of Variation (CV) and %CV for each cell density group for your data? Cell Density AVG SD CV %CV A B C D E 100,000 50,000 25,000 12,500 6,250 2. What is the equation of your regression line? 3. What is the r² value for your data? F G H 3,125 1,500 0 DIAM 10 MURSY 1010 UDW JN IDW 4. Using the values above, determine the optimal cell number to plate for cytotoxicity assay. This should be done based on the combination of two factors: 1. Low CV value 2. Linearity (r2 value)
5. Describe the reasoning behind your decision on optimal cell number to plate. 6. What are some errors that may have given you skewed results in your inoculation experiment? If you didn't have skewed results identify some parts where errors can occur. 7. For the next experiment, Cytotoxicity SRB Assay what is the 1) total number of cells you need to complete the experiment 2) total volume in mLs of media if you have: 48 wells 12,000 cells/well Final well volume of 100 uL
Transcribed Image Text:5. Describe the reasoning behind your decision on optimal cell number to plate. 6. What are some errors that may have given you skewed results in your inoculation experiment? If you didn't have skewed results identify some parts where errors can occur. 7. For the next experiment, Cytotoxicity SRB Assay what is the 1) total number of cells you need to complete the experiment 2) total volume in mLs of media if you have: 48 wells 12,000 cells/well Final well volume of 100 uL
Expert Solution
Step 1: Calculation of mean

Calculate the Mean:

Mean (μ) = (Sum of all values) / (Number of values)

μ = (100,000 + 50,000 + 25,000 + 12,500 + 6,250 + 3,125 + 1,500 + 0) / 8 μ

  = 198,375 / 8 μ

   ≈ 24,796.875

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