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When testing the sensitivity of antibiotics using the Kirby-Bauer method, the Petri Dish with Escherichia coli had the following results:
Antibiotic Zone of inhibition
Penicillin 0 mm
Ampicillin 9 mm
Ciproflaxacin 30 mm
Tetracycline 31 mm
What are the best conclusions you can make from the choices below?
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- The following results were obtained from a disk diffusion test for microbial susceptibility to antibiotics. Staphylococcus aureus was the test organism. Use these data to answer questions. The following results were obtained using the Kirby-Bauer method to assess microbial susceptibility to antibiotics. Staphylococcus aureus was the experimental organism. Use the data below to answer the questions. antibiotics Zone of Inhibition TO 3mm В. 7mm C Omm 10mm The most effective antibiotic was: Select one: O TO B. can't tell O O O O O12:28 TABLE 1 Disk Symbol A B Today 10:42 AM INTERPRETING INHIBITION ZONES OF TEST CULTURES CHEMICAL METHODS OF CONTROL: ANTIMICROBIAL DRUGS Antimicrobial Agent Ampicillin when testing gram-negative bacteria Ampicillin when testing gram-positive bacteria Chloramphenicol Ceftazidime Carbenicillin Carbenicillin when testing Pseudomonas Cephalothin Ciprofloxacin Erythromycin Cefoxitin Sulfisoxazole Bunnsoxazo Gentamicin Conca companion Imipenem Penicillin when testing staphylococci Penicillin when testing other bacteria Polymyxin Rifampin Streptomycin Trimethoprim- Sulfamethoxazole (TMP-SMZ) Tetracycline Vancomycin when testing Staphylococcus spp. Vancomycin when testing enterococci PROCEDURE Second Period 1. Measure the zones of inhibition in millimeters, using a ruler on the underside of the plate (see Figure 3b). If the diameter is difficult to measure, measure the radius from the center of the disk to the edge of the zone. Multiply the radius by 2 to get the diameter of the zone. Disk…Antibiotic Code Zone of Inhibition (mm) S, I, or R? Penicillin P 15 Streptomycin S 8 Ampicillin A 25 Chloramphenicol C 14 Bacitracin B 5 QUESTIONS: Which antimicrobial agent was most effective against your organism? Can you determine from your results whether this antimicrobial agent will also be most effective against another bacterium? Why or why not? Is this technique measuring bacteriostatic effect, bactericidal effects, or both? Explain. 4) Consider the following list of hypothetical antibiotics, tested against Escherichia coli in a Kirby-Bauer test: Antibiotic Zone of Inhibition (mm) Astonostatin 12 Bodaciosporin 25 Crazifloxacin 5 Dorkimycin 16 When the above antibiotics were tested as treatments for E. coli infections in humans, it was found that Dorkimycin has virtually no effect on the infection, while Astonostatin is the most effective of the four antibiotics listed. Propose an explanation for this result. Based purely on these…
- A relatively large zone of inhibition surrounding an antimicrobial disk on a Kirby-Bauer test plate would most likely be interpreted as: a. sensitive reaction b. intermediate reaction c. resistant reactionIf you have 4 compounds and you test them for antimicrobial activity using a disk diffusion assay and one is very active, one is moderately active, one is slightly active and one is not active at all, draw the disk, the zone of inhibition that you would see and a pretend numerical value to indicate the size of the zone in mm, give each one a number 1 – 4 and rank them from most effective to least effective as an antimicrobial.3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTES
- There are many ways you can accomplish a 1000-fold dilution. Propose 3 different serial dilution methods that will accomplish a dilution with a 1:1000 dilution factor.Ex. 17: Chemotherapeutic Agents Using the Antimicrobial Susceptibility Table on pg 62 in the lab manual, determine whether not the bacteria plated in this Kirby-Bauer test are resistant (R), intermediate (I), or susceptible (S). The bacterial species tested was Escherichia coli (Gram negative). Ciprofloxacin Diameter = 20 Streptomycin Diameter 30 Carbenicillin Diameter 17 + Erythromycin Diameter = 23 Novobiocin Diameter = 0 bacterial growth = disk with antibiotic (see labels) = no growthBased on the table, what can you conclude from the said data? Title: Indicate whether the microorganism is Resistant, Intermediate or Susceptible with the antibiotic based on the measurement of their zone of inhibition. ANTIBIOTIC MICROORGANISMS Pseudomonas aeruginosa Escherichia coli Staphylococcus aureus Amoxicillin (AMC) Resistant Resistant Resistant Cephalothin (CF) Resistant Susceptible Resistant Chloramphenicol (C) Intermediate Susceptible Resistant Ciprofloxacin (CIP) Susceptible Susceptible Resistant Clindamycin (CC) Resistant Resistant Resistant Erythromycin (E) Resistant Resistant Resistant Oxacillin (OX) Resistant Resistant Resistant Penicillin G (P) Resistant Resistant Resistant Streptomycin (S) Resistant Susceptible Resistant Tetracycline (TE) Resistant Intermediate Resistant Tobramycin (TM) Susceptible…
- How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.Which of the following is a reason to run simultaneous tests on known positive and negative controls when identifying an unknown microbe using biochemical tests? O The controls maintain the differential qualities of the test medium The controls will ensure that the unknown microbe multiplies on the medium O The controls can show if there was contamination of the tubes with another species O The controls can show if there was a recent mutation in your unknown microbe MacBook Air DII F12 80 888 F7 F5 F4 F3 * & %2$ %23 6 7 8. 4 5The following results were obtained from a disk diffusion test for a strain of Escherichia coli. Chemotherapeutic Zone of inhibition (mm) A 7 B 10 C 18 D 3 Which drug above (A, B, C, or D) is most effective against this E. coli strain? ***Which drug above (A, B, C, or D) is most effective against Staphylococcus aureus? Explain your answer.