Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
You perform the Methyl-Red(MR)& Voges-Proskauer(VP)test and gather the followingresults: (Uploaded picture).
VP test result is on the left, MR test result is on the right.
A) Please interpret these results.
B) Your fellow researcher and yourself are sure your bacterial sample is either E. coli or P. vulargis. Basedupon the work we have done in lab which methods will help you determine which bacteria it is? Please explain why, the results you would want to observe and provide analysis of those results.
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