In the ELISA, the pH the coating buffer was 9.6 whereas the pH of the sample buffer (PBS based) was 7.4 a) Why was the pH of the coating buffer so different? b) What is “coating buffer” for an ELISA? Does the buffer molecule used make sense based on the desired pH? In an ELISA procedure the samples are incubated and the ELISA plate is covered with parafilm and placed in a humidified chamber to prevent evaporation of the small liquid volumes in the wells. c) How would your results change if you did not incubate in a humidified chamber?
In the ELISA, the pH the coating buffer was 9.6 whereas the pH of the sample buffer (PBS based) was 7.4 a) Why was the pH of the coating buffer so different? b) What is “coating buffer” for an ELISA? Does the buffer molecule used make sense based on the desired pH? In an ELISA procedure the samples are incubated and the ELISA plate is covered with parafilm and placed in a humidified chamber to prevent evaporation of the small liquid volumes in the wells. c) How would your results change if you did not incubate in a humidified chamber?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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In the ELISA, the pH the coating buffer was 9.6 whereas the pH of the sample buffer (PBS based) was 7.4
a) Why was the pH of the coating buffer so different?
b) What is “coating buffer” for an ELISA? Does the buffer molecule used
make sense based on the desired pH?
In an ELISA procedure the samples are incubated and the ELISA plate is
covered with parafilm and placed in a humidified chamber to prevent
evaporation of the small liquid volumes in the wells.
c) How would your results change if you did not incubate in a humidified
chamber?
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