Answered: c) In performing an immunological…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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c) In performing an immunological procedure, you add 2 mL of stock
serum to 8 mL of saline. Two 1/4 serial dilutions are then made. What is
the dilution of serum contained in the final tube?

d) How would you perform a 1/5 dilution of glucose stock solution in a final
volume of 1 mL (using water as a diluent)? Write all the steps of the
procedure and show all your calculations.

Expert Solution

Question C

We are adding 2 ml of serum to 8 ml of saline, then the total volume will be 10 ml. Dilution factor is the ratio of total volume to sample volume that is 10/2 which is 5. We are diluting the serum by a factor of 5. So this is 1:5 dilution.
If we are doing 1/4 dilution then it means 1 part of serum is diluted in 3 parts of saline (denominator part indicates the total volume).
If we are taking 2 ml of serum then the saline volume should be 6 ml (1 part is diluted in 3 parts so the saline volume should be 6 ( 2 * 3)). This is 1st 1:4 dilution.
Here the dilution factor is 8/2 which equals 4. This is our second dilution.

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