equal volume of cell suspension + equal volume of Trypan Blue). Take this into account when determining cell number. Any dilution listed in table is an additional dilution. Live and dead cells listed below represent the total number of cells in 5 large counting squares. *1 x 105 cells/ml cells were seeded into each well of the 24 well plate(s) on day 0 to begin the growth study. Plot this value at the zero time point on your graph.
equal volume of cell suspension + equal volume of Trypan Blue). Take this into account when determining cell number. Any dilution listed in table is an additional dilution. Live and dead cells listed below represent the total number of cells in 5 large counting squares. *1 x 105 cells/ml cells were seeded into each well of the 24 well plate(s) on day 0 to begin the growth study. Plot this value at the zero time point on your graph.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Please note that these counts have all been done with Trypan Blue. Therefore, all cultures have been diluted two-fold (equal volume of cell suspension + equal volume of Trypan Blue). Take this into account when determining cell number. Any dilution listed in table is an additional dilution. Live and dead cells listed below represent the total number of cells in 5 large counting squares.
*1 x 105 cells/ml cells were seeded into each well of the 24 well plate(s) on day 0 to begin the growth study. Plot this value at the zero time point on your graph.
1These numbers represent the total number of cells counted in 5 large squares from three separate counts.
Transcribed Image Text:Total
Volume
Live
Cells/ml
(live cells only)
Day
Hemo-
Dilution
Dead
%
cytometer
Viability
Neubauer
1ml
NA
NA
NA
95
1 x 105
1
Neubauer
1ml
30
2
2
Neubauer
1ml
60
1
Neubauer
1ml
95
1
4
Neubauer
1ml
185
3
6.
Neubauer
1ml
2
376
8.
Neubauer
1ml
587
10
10
Neubauer
1ml
25
464
12
12
Neubauer
1ml
25
519
30
14
Neubauer
1ml
25
503
80
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