You are testing a sample of water from a swimming pool. You count a plate made from a 1 mL transfer of a 10-1 dilution and it has 38 colonies on it. Calculate the number of CFu/mL for this plate.
You are testing a sample of water from a swimming pool. You count a plate made from a 1 mL transfer of a 10-1 dilution and it has 38 colonies on it. Calculate the number of CFu/mL for this plate.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
![### Calculating CFUs from a Plating Experiment
You are testing a sample of water from a swimming pool. You count a plate made from a 1 mL transfer of a 10⁻¹ dilution and it has 38 colonies on it. Calculate the number of CFU/mL for this plate.
#### Options:
1. ( ) 3.8 x 10⁻¹
2. ( ) 3.8 x 10¹
3. ( ) 3.8 x 10⁻²
4. ( ) 3.8 x 10²
5. ( ) 3.8 x 10⁰
To calculate the original concentration (CFU/mL) of the swimming pool water sample, follow these steps:
1. **Count the number of colonies on the plate**: 38 colonies.
2. **Note the dilution factor used**: 10⁻¹.
3. **Use the formula for CFU/mL**:
\[ \text{CFU/mL} = \frac{\text{Number of Colonies}}{\text{Volume Plated} \times \text{Dilution Factor}} \]
In this case:
- Number of Colonies = 38
- Volume Plated = 1 mL
- Dilution Factor = 10⁻¹
\[ \text{CFU/mL} = \frac{38 \text{ colonies}}{1 \text{ mL} \times 10⁻¹} \]
\[ \text{CFU/mL} = \frac{38}{0.1} \]
\[ \text{CFU/mL} = 38 \times 10 \]
\[ \text{CFU/mL} = 3.8 \times 10^2 \]
Thus, the correct option is:
4. (X) 3.8 x 10²](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F9479e5a3-d081-4d60-94d0-d376a9c3f85d%2F935aee3b-92f6-4c42-9e54-5a8fc7161114%2Fpgi8d6l.jpeg&w=3840&q=75)
Transcribed Image Text:### Calculating CFUs from a Plating Experiment
You are testing a sample of water from a swimming pool. You count a plate made from a 1 mL transfer of a 10⁻¹ dilution and it has 38 colonies on it. Calculate the number of CFU/mL for this plate.
#### Options:
1. ( ) 3.8 x 10⁻¹
2. ( ) 3.8 x 10¹
3. ( ) 3.8 x 10⁻²
4. ( ) 3.8 x 10²
5. ( ) 3.8 x 10⁰
To calculate the original concentration (CFU/mL) of the swimming pool water sample, follow these steps:
1. **Count the number of colonies on the plate**: 38 colonies.
2. **Note the dilution factor used**: 10⁻¹.
3. **Use the formula for CFU/mL**:
\[ \text{CFU/mL} = \frac{\text{Number of Colonies}}{\text{Volume Plated} \times \text{Dilution Factor}} \]
In this case:
- Number of Colonies = 38
- Volume Plated = 1 mL
- Dilution Factor = 10⁻¹
\[ \text{CFU/mL} = \frac{38 \text{ colonies}}{1 \text{ mL} \times 10⁻¹} \]
\[ \text{CFU/mL} = \frac{38}{0.1} \]
\[ \text{CFU/mL} = 38 \times 10 \]
\[ \text{CFU/mL} = 3.8 \times 10^2 \]
Thus, the correct option is:
4. (X) 3.8 x 10²
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education