You plated 0.1 ml of a sample of kombucha diluted by a factor of 10^-3. It produced 43 colonies. What was the original concentration in the sample? Show your calculations.

Q: A food sample with an unknown number of contaminating bacteria was serially diluted to 10-6. A…

A: Serial dilution of a food sample containing unknown quantity of contaminating bacteria was performed…

Q: Calculate the CFU/ml of leuconostoc in the brine for the 4 different days. Show your calculation

A: CFU/ml stands for "colony forming units per milliliter." It is a unit of measurement used in…

Q: My question is: Could you explain steps? (etc. why do we heat or add chemicals like nacl or ammonium…

A: Casein is the major protein component found in milk. It is generally a phosphoprotein which is…

Q: What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution and added it to a…

A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…

Q: In a plate count, 1 mL of culture is spread on a plate and incubated for 24 hours. 250 colonies are…

A: Given,number of colonies in a 1mL of culture = 250. Also,given that this given solution is 100 times…

Q: How to count the number of colonies forming units per milliliter (CFUs per ml) of the sample?

A: A viable cell is one that has the ability to divide and form a colony. The colony-forming unit (CFU)…

Q: You make a 1:100 dilution of your culture and plated 0.1 ml of this sample on a Nutrient agar plate.…

A: The amount of organisms present in the original culture is called as colony forming unit per…

Q: Your broth culture contains 471,612,048 bacteria/mL. Design a dilution series that would  result in…

A: To ensure an accurate count of bacterial colonies on agar plates, it's crucial to design a dilution…

Q: A microbiology student was given a mixed culture of two different gram positive bacteria species to…

A: In Microbiology, we grow bacteria on culture medium. Culture media contains specific mixture of…

Q: You are performing an enzyme reaction that works the same way as your wheat germ acid phosphatase…

A: Enzymes can be defined as proteins that act as biological catalysts. They work by accelerating…

Q: You prepared 10-7, 10-8, and 10-9 dilutions of a bacterial suspension in sterile saline. Then you…

A: Introduction There are different culturing techniques such as agar plates, broth culture, stab…

Q: Jessie is working with a 100% stock concentration of Clorox. She decides to add 100 μL of bacteria…

A: For calculating the unknown concentration of the tube we will be using the formula as mentioned…

Q: Using a dilution series is a way to transfer the very small volumes of original culture necessary to…

A: Answer -To determine the volume of the original bacterial culture required to obtain a specific…

Q: CFU

A: CFU:It stands stands for Colony-Forming Unit. It is a measure used to estimate the number of viable…

Q: Determine the titer for a plate made using milk and labeled 10^-6. you count 50 colonies.

A: Introduction:-  To Calculate the number of bacteria per milliliter or gram of a sample it is  divide…

Q: Dilution Problem. A culture was diluted as follows: (1) 50mL are added to 100mL of water. (2) 1mL…

A: It refers to the process of reducing the concentration of a substance in a solution by adding more…

Q: An original sample of water containing 4.20 X 109 CFU/mL was diluted by 4 successive 1/100…

A: Serial dilution is used to find the concentration of bacteria in the sample in case of greater…

Q: What do to do when the disstillation did not make the sample completely pure? What could have went…

A: Distillation is a process that is based upon the boiling points of various liquids present in the…

Q: You want to subculture your cells from T25 flask to 96-well plates. You first collected your cells…

A: A hemocytometer is an instrument that is commonly used to count the number of cells present in a…

Q: A scientist transfers 0.1 mL phage stock to a tube containing 9.9 mL tryptic soy broth. She then…

A: The total fold of dilution represents how much the original concentration has been reduced due to…

Q: Suppose your professor handed you a bottle of kombucha with 2.0 ml in it and told you to make a…

A:

Q: Why is UV light used in Column chromatography experiment ?

A: A chromatography procedure used to separate a single chemical compound from a mixture is known as…

Q: You saw no color change in both the first step and second step of the Nitrate Reduction test. What…

A: A nitrate reduction test is a test done to test whether the Enterobacteriaceae member produces the…

Q: From a water sample, you must determine the number of colonies present (CFU's/ml.). However, looking…

A: Counting with the CFU colony-forming units needs culturing the microbes. It counts only the viable…

Q: You obtain a 1 mL sample of contaminated drinking water in order to calculate the number of  fecal…

A: The evaluation of fecal microscopic organisms in water could be a critical public well-being measure…

Q: ) If you counted 500 CFU’s in the petri dish for number 6 and 5000 CFU’s in petri dish for number 7,…

A: See below section.Explanation:Detailed explanation:a): Let's break down the situation and explore…

Q: A tampered sack of powdered milk was analyzed by getting 50 grams of composited sample and diluting…

A: Dilution : We know that the solute of the concentration will be decreasing in solution . There is a…

Q: step by step process of Methyl Red test using a schematic diagram

A: The Methyl Red Voges Proskauer protocol is used to identify and differentiate the microbes based on…

Q: You perform several tests on an unidentified bacterial species and get the results below. Based on…

A: Bacterial identification is the process of determining the specific type, species, or strain of…

Q: You perform a serial dilution of a water sample and the 10-7 tube when 0.1 ml was plated onto the…

A: Estimation of the number of viable cells is important in microbiology protocols. It is estimated…

Q: You plated 1.0 mL of a sample diluted by a factor of 10^-5 and you counted 50 comonies after…

A: The stock concentration of the bacteria can be determined by counting the colonies formed on the…

Q: A 10-5 dilution is performed on a culture of bacteria in order to perform viable plate counts. From…

A: Serial dilution is a process by which a substance such as a bacterial culture is diluted stepwise in…

Q: You perform a set of serial dilutions using 99-mL water blanks (successive 1-mL dilutions into…

A: Colony forming units (CFUs) are used to determine the concentration of viable microorganisms in a…

Q: You have several different media onto which you inoculated eight strains of yeast (A-H). The media…

A: Minimal medium (unsupplemented) A minimal medium is a growth medium used for growing microbes and…

Q: On the basis of the appearance of this place, what are you testing for? When you added a reagent to…

A: Catalase is an enzyme that reacts with hydrogen peroxide to form water and oxygen. Catalase enzyme…

Q: Mary wanted to know the amount of microbes in her garden. To do this, she took a 10g soil sample,…

A: Serial dilution is a process done to reduce the microbial count in the sample. This is specifically…

Q: A stock solution of a bacterial strain is diluted 1/10000 to a total volume of 500ul. Then, 50ul of…

A: The mixing of the stock solution with the dilutant liquid is known as dilution. The ratio of stock…

Q: A 10-2 is performed on a culture of bacteria in order to perform viable plate counts. From the…

A: CFU means colony forming unit. It is a measure of viable bacterial or fungal cells. In case of…

Q: You are determining the concentration of your culture, and have counted the following on your…

A: Hemocytometer is a glass slide at with margins on its surface which is used for counting the number…

Q: Can you show the step by step process of Voges Proskauer Test using a schematic diagram?

A: Voges Proskauer(VP) Test is a biochemical method of detecting the presence of intestine-inhabiting…

Q: Jessie is working with a 100% stock concentration of Clorox. She decides to add 100 µL of bacteria…

A: The scenario involves working with a 100% stock concentration of Clorox, bacteria, and a nutrient…

Q: How many bacteria would be in 1ml of the sample if you counted 134 colonies on the 1:1,000 dilution?

A:

Q: The stool samples submitted by the two friends were enumerated following enrichment. The technician…

A: The objective of this question is to calculate the bacterial concentration in the enriched culture…

Q: You are interested in the total bacterial load of the bat guano. In order to determine this, you go…

A: For calculation of total bacterial count we choose the solution that usually gives the colony number…

Q: Design a serial dilution of a 25-gram cheese sample to achieve a final dilution of 10-5 using 90 ml…

A: In a Series Dilution or sequential dilution the concentration decreases by the same factor at each…

Q: A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it.…

A: A serial dilution is a process of sequential dilution in order to decrease the density of cell…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.