A sample of pond water is tested using the most probable number method. There are 3 positive tubes for 10 mL, 1 positive thbe for 1 mL, and 1 positive tube for 0.1 mL. the most probable number per 100 mL is ________ ? fill in the blank.
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A sample of pond water is tested using the most probable number method. There are 3 positive tubes for 10 mL, 1 positive thbe for 1 mL, and 1 positive tube for 0.1 mL. the most probable number per 100 mL is ________ ?
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- A test procedure calls for 5 mL of undiluted specimen. 2 mL of a 1:50 dilution is used. The answer obtained is 30 mg/dL. What answer should be reported?To determine the cfu/ml of a pondwater sample, you perform 1/10, 1/100, 1/1000 and 1/10,000 serial dilutions and plate 0.1 mls of each dilution. You count 40 colonies on the plate derived from the 1/10,000 dilution. What is the cfu/ml of the pondwater? Show your work and use scientific notation.A plate has 72 colonies with a total dilution factor of 10-7, 0.1 ml was pipetted onto the plate, what was the original CFU/ml concentration of that sample?
- Which among the following statements is correct? O A positive result for Fohl's test is the formation of a black precipitate in the form of lead. O A positive result for Millon's test is characterized by the formation of a red-colored precipitate or solution. O Both statements are correct Both statements are incorrectUsing 10 μL of a patient's serum, how much diluent would be needed to create a 1:10 dilution? ______ μLA sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it. Assuming you plated the same volume on each plate, how many colonies would you expect to find on the 10^-4 plate? Include units and use OCD formula.
- The table below summarizes the results for Lead-sulfide test. Provide the correct remarks from the results if Negative for Lead-sulfide test or Positive for the Lead sulfide test and it's either Negative for -SH or Positive for -SHHow many CFU per ml are there in the original sample using the following dilution and platingseries? Make sure to label all dilutions that were done.To the right is an image of a dilution that was performed. The volume above the top arrow indicates the volume that should be transferred to the next tube (i.e. Tube 1). The volume listed at the bottom-right of tube 1 indicates the volume of diluent that should be added to that tube. The stock concentration is 50μg/ml and you want to make a solution in tube 1 with a concentration of 0.4μg/ml and a total volume of 3ml. Stock ?ml Tube 1 ?ml a. How many milliliters of stock solution needs to be added to Tube 1? Round your answer to three decimal places (e.g. 0.111). b. How many milliliters of diluent needs to be added to Tube 1? Round your answer to three decimal places.
- What is the minimal concentration (MIC) for disinfectant number 1? Just write the corresponding number including the decimal as written. ug/mL. Disinfectant 1 2 3 4 concentration 0.1 ug/mL +++ ++++ ++ +++ 0.2 ug/mL ++ ++ + ++ 0.5 ug/mL + + 1.0 ug/mL 5.0 ug/mL subculture 1 2 3 4 0.1 ug/mL ++++ ++++ ++++ ++++ 0.2 ug/mL ++++ ++++ ++++ ++++ 0.5 ug/mL ++++ ++++ ++++ 1.0 ug/mL ++++ 5.0 ug/mL Submit responsePrepare 50 plates of Sheep's Blood Agar using 100mm petri dish. Add 10% of blood to the media. (Manufacturer's instructions: Suspend 38 g of blood agar base dehydrated powder in 1 liter of distilled or deionized water.) Pour 25 mL of prepared medium per plate. Express answer to the nearest whole number. How many grams of the dehydrated powder should be weighed? Answer: What is the volume of distilled water that should be used in the preparation? Answer: What is the volume of sheep's blood that should be added to the sterilized and cooled blood agar base? Answer:Choose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possible