Refer to the following diagram: 100 ul 100 μL 100μι 10 μι 10 μι 100 μι 100 μL Volume to transfer 900 uL in each tube EColl culture 10 10 10 10 10 10 Dilutions Volume of the diluted sample plated 100 ul 100 ul 100 ul 100 uL 10 ml Plate Volume of the original sample 10 ml plated 10 ml 16 ml Plate D Plate E Plate G Given that Plate D has too many colonies to count, and Plate G has no colonies at all as a result of serial dilution, draw out all four plates and demonstrate what they might look like (assuming serial dilution has been performed correctly). Based on your drawings, identify the number of CFU/ml for Plate E and Plate F. Show your calculations.
Refer to the following diagram: 100 ul 100 μL 100μι 10 μι 10 μι 100 μι 100 μL Volume to transfer 900 uL in each tube EColl culture 10 10 10 10 10 10 Dilutions Volume of the diluted sample plated 100 ul 100 ul 100 ul 100 uL 10 ml Plate Volume of the original sample 10 ml plated 10 ml 16 ml Plate D Plate E Plate G Given that Plate D has too many colonies to count, and Plate G has no colonies at all as a result of serial dilution, draw out all four plates and demonstrate what they might look like (assuming serial dilution has been performed correctly). Based on your drawings, identify the number of CFU/ml for Plate E and Plate F. Show your calculations.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:Refer to the following diagram:
100 ul
100 uL 100 pl 100 µL 100 pl 100 pL 100 pl
Volume to
transfer
A B C D E F G
900 uL in each tube
10
102 10
10
10
10
10
Dilutions
E.CoN
culture
Volume of the
diluted sample
plated
100 ul
100 μL
100 pL
100 ul
Volume of the
10 ml
10 ml
original sample
plated
10 ml
10 ml
Plate D
Plate E
Plate F
Plate G
Given that Plate D has too many colonies to count, and Plate G has no colonies at all as a result of
serial dilution, draw out all four plates and demonstrate what they might look like (assuming serial
dilution has been performed correctly).
Based on your drawings, identify the number of CFU/ml for Plate E and Plate F.
Show your calculations.
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