Graph of Absorbance vs Time(mins) 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 70 80 Time (mins) Group 3 (Ampicillin) Group 3(Control) Absorbance(60onm)
Graph of Absorbance vs Time(mins) 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 70 80 Time (mins) Group 3 (Ampicillin) Group 3(Control) Absorbance(60onm)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question
Write a short discussion based on the graph
Transcribed Image Text:Procedure
A
Add
ul
ampicillin to
50
of
Take
mixture
Mix
5ml
3
ml
Top up to 50ml.
the
overnight culture
with 45 ml LB
broth
to + Add fresh
the
LB
mixture
analyse
both
OD600
Withdraw 3ml mixture to
The mixture is
incubated
incubator at 37°c 200rpm
then
Measure every 15
mins. Stop until
75 mins.
Overnight culture
analyse (OD600)record as
zero time-point.
at
shaked
(E-coli + LB borth)
Mix
overnight culture
Sml
Take
3
ml
Add 50 ul
of
mixture
to
No ampicilin
ampicillin to
the
B (control)
with 45 ml LB
analyse
the
mixture
broth
OD600
The
mixture
is
then
Withdraw 3ml mixture to
Measure every 15
mins. Stop until
75 mins.
- analyse (OD600)record as
zero time-point.
- incubated
at
shaked
incubator at 37°c 200rpm
Graph of Absorbance vs Time(mins)
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
10
20
30
40
50
60
70
80
Time (mins)
Group 3 (Ampicillin)
Group 3(Control)
Absorbance(6oonm)
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