Why is it important to limit the quantity of cells used to prepare a smear?  Mark all that apply: 1. So that cells are not clumped and don't entrap stain creating erroneous results  2. So that the cells are spread out enough that cell morphology can be discerned 3. So that there are small groups of cells clumped together to make them visible 4. So that no contaminants are introduced onto the slide by being entrapped in clumps 5. So that the cells are spread out enough that the arrangement can be observed

Q: Peptidoglycan on bacterial cell walls is stained by crystal violet dye and for this reason those…

A: True

Q: Determine what percentage of the culture was living (viable) and what percentage was dead…

A: The word "CFU" in microbiology refers to a colony-forming unit, which is used to estimate how many…

Q: layer

A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…

Q: Which of the following describes the usefulness of a Gram stain.  Select all that apply. a. A Gram…

A: A popular microbiological method called "Gram staining" is used to separate and categorize bacteria…

Q: Select all methods that can be used disinfecting medical devices. Thermal UV Chemical Steam…

A: Disinfection is the process of  eliminating the pathogenic organism from the surface except some…

Q: Why bleach disinfectant will the most effective against probiotic?

A: Probiotics are live microorganisms that have health benefits when consumed. These are usually…

Q: Acid-Fast stain A) E. Coli -Morphology shape -Arragement -Acid-fast or non-Acid-fast B) S.…

A: Acid-fast Staining :  When the smear is stained with the primary stain carbol fuchsin, it passes…

Q: 3 things about each one? are they selective , differential , positive , negative results etc.…

A: Selective media will be used for growth of only selected microorganisms. Differential media are…

Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…

A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…

Q: List five (10) sites on a patient from which potential pathogens could be isolated and prepared for…

A: Microorganisms such as bacteria, viruses, fungi, or parasites enters the body through various sites…

Q: Dilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of the old culture…

A: Introduction Dilution is the process of lowering the concentration of a solute in a solution by…

Q: What is the purpose of a streak plate and how do you know one was successful? Explain.

A: In microbiology, the Streak plate method is used to get pure isolated colonies. As we know that a…

Q: Gram staining is the most common initial test of unknown identification, explain in your own words…

A: The Gram staining is a typical process that is used to separate two groups of microorganisms based…

Q: The acid-fast stain is used to stain _____. The decolorizer used to destain the cells is ______.…

A: The staining procedures used to differentiate the types of bacteria are known as a differential…

Q: e the process to make a 4-phase streak plate beginning with your first streak (i.e you have…

A: The sample/inoculum is usually pored by streak with the diluted through streaking it across the…

Q: Define smear, simple stain, direct stain, negative stain What information is provided by a simple…

A: Microbes are microscopic structure that cannot be seen as such through naked eyes but require…

Q: colony-forming units

A: Colony Forming Unit:It is designated as CFU.It can defined as the unit of measurement used to…

Q: Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and…

A: Bacteria are small, microscopic and unicellular organisms that can live in variety of environment.…

Q: please label this in the correct order for gram staining.  crystal violet, iodine, alcohol, and…

A: Gram staining is a method of staining useful for identifying and classifying bacteria into two major…

Q: When viable cell concentrations are too high to count on an agar plate, it is common to use larger…

A: The correct option is shown below.

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.