Answered: colony-forming units

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

Ten grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of a
mL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further diluted
by successive 1/100 and 1/10 dilutions. One-tenth of a mL of this final solution was plated onto Plate Count agar.
After incubation 145 colonies were present. How many colony-forming units were present in the total 10 gram
sample of hamburger?

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