A 5mL sample diluted 1:1 with trypan blue is counted using a hemocytometer. A total of 325 viable cells and 52 dead cells were counted in the four corner square and the big central square of the meter. What is the total number of dead cells per mL of the sample?

Q: Dr. Riggs took 1ml of whole mouse blood and added it to 10ml of PBS. You next take 0.5 mL of Dr.…

A: The first step is to calculate the average number of cells counted in the hemacytometer. The counts…

Q: Why does counting 5 Boxes in each chamber of the hemacytometer gives you the number of cells in a ul…

A: Hemacytometer is a medical instrument used to measure the concentration of blood components in a…

Q: You have performed a serial dilution of an unknown sample and counted 73 CFU on a countable plate…

A: Serial dilution is method of diluting a bacterial culture in succession. The process reduces the…

Q: You have added 8 mL of Albuterol Sulfate Solution (5mg/mL) and 22 mL of normal saline to your…

A: Introduction:-  Albuterol sulfate solution is in concentration of 5mg/ml and total of 8ml is to…

Q: One ml of a water sample was placed in a Petri dish with melted agar. After incubation, 129 colonies…

A: A colony-forming unit, or CFU, is a measurement of the concentration of microorganisms in a test…

Q: 10ul of the original sample was transferred to 90ul diluent. Then, the mixture was mixed with 100ul…

A: Hemocytometer is blood cell counting device. It is usally cposed of thick glass on which square…

Q: What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution and added it to a…

A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…

Q: Calculate the final dilution in the following dilution series:  a. 1 mL in  9 mL dilution & 100uL…

A: Dilution is calculated as amount of sample transfered into the diluent divided by the total amount…

Q: This is the growth curve for Clostirdium (incubated under optimal growth conditions). Use it to…

A: There are 5 phases to a logistic (sigmoidal) growth curve.  They are stationary phase, lag phase,…

Q: Please count the cells in grids 1, 3, 7, and 9. Calculate the cell density (a) and cell viability…

A: The hemocytometer was first discovered by Louis Charles Malassez to count blood cells, hence, the…

Q: Steven is a lab research assistant. He mixed 25 µL of cells with 50 µL of cell culture media in…

A: A Hemocytometer slide is a specially designed glass slide with Neubar's rulings in the central…

Q: A total of 1,000 cells were counted from 5 squares of the hemocytometer. Of these, 20 are trypan…

A: Hemocytometer is a device which is made up of a glass which is thick and a microscopic slide that…

Q: A further sample of blood was taken from the above individual and prepared for haemoglobin analysis.…

A: The volume of a blood sample taken = 18 ml Volume of anticoagulant = 2 ml Out of this 20 ml, 5 ml…

Q: Prepare 50 plates of Sheep's Blood Agar using 100mm petri dish. Add 10% of blood to the media.…

A: Sheep Blood Agar Base was formed to be compatible with sheep blood and to improve organism…

Q: Jessie is working with a 100% stock concentration of Clorox. She decides to add 100 μL of bacteria…

A: For calculating the unknown concentration of the tube we will be using the formula as mentioned…

Q: You have a 1:5 dilution. Describe how you would make a 1:100 dilution.  When 0.5 µL of serum is…

A: DF=Volume of final diluted solutionVolume removed from stock and added to new dilution   Dilution…

Q: You aseptically transfer 1 ml of your optional liquid culture into 99 ml of sterile water. What is…

A: The following are needed for calculating the dilution factor  1) Original volume of  solution we…

Q: As shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the…

A: The number of microorganisms that are able to divide and form new colonies is called a…

Q: If each agar plate contained 20 ml of LB agar and had an ampicillin concentration of 150 ug/ml, what…

A: Given data:  Each plate contains-  LB agar=20ml Ampicillin= 150 micrograms per ml.  Amount of…

Q: You have 0.05 ml of kombucha that contains microbes at a concentration of 8.2 x 10^6 CFU/ml. You…

A: Creating proper dilutions and working out the colony-forming units or CFU/ ml is one integral part…

Q: You perform a series of 4 different dilutions of 200 µL of sample into 800 µL of saline. What is the…

A: Dilution factor can be calculated using the formula:C1V1=C2V2

Q: If you performed the serial dilution using 0.9ml of tapwater originally and mixed and transferred…

A: A serial dilution is one of the techniques which is useful in microbiology, serology, and other…

Q: You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell…

A: Hemocytometer is device used for the counting of blood cells in a sample. It has counting chambers…

Q: If an OD=1.0 culture yielded a plate count of 2.2 x 107 cfu/mL. What is the cell count of an OD=0.5…

A: Answer: The relationship between colony-forming units per millilitre (cfu/mL) and optical density…

Q: 1 ml of urine is added to 2 ml of water. 1 ml is plated. 81 colonies form. What is the CFU/ml of the…

A: CFU/ml is the indirect measurement of the microbes present in the sample. It can be related to…

Q: If the 10-9 plate was counted to have 155 colonies on it, would the CFU/mL be 1.55*1011? If not,…

A: CFU  Colony forming unit it a method to calculate the number of microorganisms in a culture media.

Q: ) If you counted 500 CFU’s in the petri dish for number 6 and 5000 CFU’s in petri dish for number 7,…

A: See below section.Explanation:Detailed explanation:a): Let's break down the situation and explore…

Q: You have been asked to determine the bacterial count in a sample of water. The number of CFU from 1…

A: The CFU count is the number of viable cells per ml of the sample. The CFU count is done on the basis…

Q: A solution of flycine and a solution of albumin were prepared with the same concentration (g/L). One…

A: The ninhydrin test is a chemical that is used to determine the presence of alpha amino groups in the…

Q: Propose a serial dilution that would give a dilution of 1/153 000 000. Each tube in your dilution…

A: The dilution factor after four dilutions is DF= 1/153000000 If the concentration of the original…

Q: Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you…

A: Cfu refers to the colony forming unit which is formed through continuous replication of a single…

Q: A milk sample was tested for the presence of typhimurium. Using a 10 microliter calibrated loop, a…

A: Serial dilution is the technique to find the concentration of bacteria in a sample when there are…

Hematology

Hematology is the branch of biology. Hematology is the medical field that deals with the treatment of blood disorders and cancers, such as hemophilia, leukemia, lymphoma, and sickle-cell anemia. Hematologists and hematopathologists are medical professionals who specialize in illnesses of the blood and its components.