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- The decolorizing agent damages the peptidoglycan layer. Why is this important to consider when deciding how long to decolorize a specimen as part of the
Gram stain procedure?
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- For a laboratory exercise of basic techniques in microbiology: Gram stain and Microscopy. What are the sources of error in performing Gram staining?List the reagents of the Gram stain technique in order and their general role in the staining process.After performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple bacteria. Which of the following could notexplain why this occurred? Decolorizer was used after safranin Safranin was not used Crystal violet was not used The specimen was not heat-fixed
- List the step-by-step procedure of Gram staining.The acid-fast bacterium, Mycobacterium smegmatis, is relatively safe to be used by students in performing the acid-fast stain technique because of its thin cell wall. However, if this organism is used for Gram-stain, it can also take the Gram stain reaction. Why is this so?Explain how bacterial cells would look in the gram staining procedure if the following mistakes were made: a. Decolorizer left on too long b. Decolorizer not left on long enough c. Slide not heat-fixed before staining
- You receive a bacterial culture in the lab and your job is to figure out information which will be helpful. You are only allowed to use one of the staining methods we practiced. Which one would be the best option? Why? And what will this information help you determine? Explain.In Gram staining method, list the reagents and state their role for each step of the procedure? In preparing bacterial smear, why do we need to pass the slide over the flame?Which of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.
- Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?Briefly, in your own words, describe the steps of the gram staining procedure.Describe the color and appearance of a Gram positive and Gram negative bacteria as it is treated at every step of the Gram staining procedure