Which of the following serves as a counterstain for the gram-stain technique? It also happens to be the counterstain for the endospore staining technique.
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Which of the following serves as a counterstain for the gram-stain technique? It also happens to be the counterstain for the endospore staining technique.
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- Why is it essential that the primary stain and the counterstain be contrasting colors in gram-staining?Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayList the step-by-step procedure of Gram staining.
- You receive a bacterial culture in the lab and your job is to figure out information which will be helpful. You are only allowed to use one of the staining methods we practiced. Which one would be the best option? Why? And what will this information help you determine? Explain.Which of the following are true about a differential stain. There may be more than one answer! No chemicals are needed. More than one stain is used The acid fast stain is an example The Gram stain is an example.What staining technique is used here? What is the primary stain, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with the blue color. Describe the organism with the bright pink color. Name the genus of bacteria with bright pink.
- List the reagents of the Gram stain technique in order and their general role in the staining process.For a laboratory exercise of basic techniques in microbiology: Gram stain and Microscopy. What are the sources of error in performing Gram staining?Which step of the gram stain procedure is most critical?why?
- You have performed an experiment and your aim is to obtain pure colonies. However, you observe some colony variations and you need to verify that this is not due to contamination, name and describe the staining method would you use to achieve this goal?Define the term gram staining.A mixed culture is known to contain Gram negative bacilli and Gram positive cocci. You perform a Gram stain of this mixed culture as a control while simultaneously Gram stainin an unknown organism on the same slide (the same procedure you used in lab). The results, observed under the 100x objective lens, are described below: Results: Control side: purple bacilli and purple cocci Unknown side: purple bacilli Using all the information given above, discuss the results of your unknown organism. Shoul you be confident in this result? Answer question A if you are confident, answer question B if you are not confident. a. If you are confident in your results, why? Explain how the staining reagents produced the described results. b. If you are not confident, do you think an error was made in the Gram staining procedure? If so, describe the error and explain how it impacted your results.