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If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is completed? Crystal violet Safranin Decolorizer lodine

If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is completed? Crystal violet Safranin Decolorizer lodine

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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### Gram Staining of Gram-Negative Bacteria **Question:** If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is completed? **Options:** - [ ] Crystal violet - [ ] Safranin - [ ] Decolorizer - [ ] Iodine In this multiple-choice question, students are asked to identify the dye that will be visible after a gram-negative bacterium is stained using the Gram staining technique. 1. **Crystal violet:** This is a primary stain used in Gram staining. Although it initially stains all bacteria, it is not retained by gram-negative bacteria after the decolorization step. 2. **Safranin:** This is a counterstain applied after decolorization. It stains the gram-negative bacteria pink or red. 3. **Decolorizer:** This is usually an alcohol or acetone solution that removes the crystal violet stain from gram-negative bacteria. 4. **Iodine:** This is a mordant applied after the crystal violet. It forms a complex with crystal violet that helps retain the stain in gram-positive bacteria but not in gram-negative bacteria. **Correct Answer:** - [ ] Safranin
Transcribed Image Text:### Gram Staining of Gram-Negative Bacteria **Question:** If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is completed? **Options:** - [ ] Crystal violet - [ ] Safranin - [ ] Decolorizer - [ ] Iodine In this multiple-choice question, students are asked to identify the dye that will be visible after a gram-negative bacterium is stained using the Gram staining technique. 1. **Crystal violet:** This is a primary stain used in Gram staining. Although it initially stains all bacteria, it is not retained by gram-negative bacteria after the decolorization step. 2. **Safranin:** This is a counterstain applied after decolorization. It stains the gram-negative bacteria pink or red. 3. **Decolorizer:** This is usually an alcohol or acetone solution that removes the crystal violet stain from gram-negative bacteria. 4. **Iodine:** This is a mordant applied after the crystal violet. It forms a complex with crystal violet that helps retain the stain in gram-positive bacteria but not in gram-negative bacteria. **Correct Answer:** - [ ] Safranin
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