If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is completed? Crystal violet Safranin Decolorizer lodine
If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is completed? Crystal violet Safranin Decolorizer lodine
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
![### Gram Staining of Gram-Negative Bacteria
**Question:**
If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is completed?
**Options:**
- [ ] Crystal violet
- [ ] Safranin
- [ ] Decolorizer
- [ ] Iodine
In this multiple-choice question, students are asked to identify the dye that will be visible after a gram-negative bacterium is stained using the Gram staining technique.
1. **Crystal violet:** This is a primary stain used in Gram staining. Although it initially stains all bacteria, it is not retained by gram-negative bacteria after the decolorization step.
2. **Safranin:** This is a counterstain applied after decolorization. It stains the gram-negative bacteria pink or red.
3. **Decolorizer:** This is usually an alcohol or acetone solution that removes the crystal violet stain from gram-negative bacteria.
4. **Iodine:** This is a mordant applied after the crystal violet. It forms a complex with crystal violet that helps retain the stain in gram-positive bacteria but not in gram-negative bacteria.
**Correct Answer:**
- [ ] Safranin](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F27937a9a-6c56-4023-8617-cb9097cc0b1e%2F3dc665d9-4af0-4994-b51e-6a13142e8d48%2Fybjuz2a_processed.png&w=3840&q=75)
Transcribed Image Text:### Gram Staining of Gram-Negative Bacteria
**Question:**
If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is completed?
**Options:**
- [ ] Crystal violet
- [ ] Safranin
- [ ] Decolorizer
- [ ] Iodine
In this multiple-choice question, students are asked to identify the dye that will be visible after a gram-negative bacterium is stained using the Gram staining technique.
1. **Crystal violet:** This is a primary stain used in Gram staining. Although it initially stains all bacteria, it is not retained by gram-negative bacteria after the decolorization step.
2. **Safranin:** This is a counterstain applied after decolorization. It stains the gram-negative bacteria pink or red.
3. **Decolorizer:** This is usually an alcohol or acetone solution that removes the crystal violet stain from gram-negative bacteria.
4. **Iodine:** This is a mordant applied after the crystal violet. It forms a complex with crystal violet that helps retain the stain in gram-positive bacteria but not in gram-negative bacteria.
**Correct Answer:**
- [ ] Safranin
Expert Solution
![](/static/compass_v2/shared-icons/check-mark.png)
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 2 steps
![Blurred answer](/static/compass_v2/solution-images/blurred-answer.jpg)
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Recommended textbooks for you
![Human Anatomy & Physiology (11th Edition)](https://www.bartleby.com/isbn_cover_images/9780134580999/9780134580999_smallCoverImage.gif)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
![Biology 2e](https://www.bartleby.com/isbn_cover_images/9781947172517/9781947172517_coverImage_Textbooks.gif)
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
![Anatomy & Physiology](https://www.bartleby.com/isbn_cover_images/9781259398629/9781259398629_smallCoverImage.gif)
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
![Human Anatomy & Physiology (11th Edition)](https://www.bartleby.com/isbn_cover_images/9780134580999/9780134580999_smallCoverImage.gif)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
![Biology 2e](https://www.bartleby.com/isbn_cover_images/9781947172517/9781947172517_coverImage_Textbooks.gif)
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
![Anatomy & Physiology](https://www.bartleby.com/isbn_cover_images/9781259398629/9781259398629_smallCoverImage.gif)
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
![Molecular Biology of the Cell (Sixth Edition)](https://www.bartleby.com/isbn_cover_images/9780815344322/9780815344322_smallCoverImage.gif)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
![Laboratory Manual For Human Anatomy & Physiology](https://www.bartleby.com/isbn_cover_images/9781260159363/9781260159363_smallCoverImage.gif)
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
![Inquiry Into Life (16th Edition)](https://www.bartleby.com/isbn_cover_images/9781260231700/9781260231700_smallCoverImage.gif)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education