If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is completed? Crystal violet Safranin Decolorizer lodine
If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is completed? Crystal violet Safranin Decolorizer lodine
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
![### Gram Staining of Gram-Negative Bacteria
**Question:**
If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is completed?
**Options:**
- [ ] Crystal violet
- [ ] Safranin
- [ ] Decolorizer
- [ ] Iodine
In this multiple-choice question, students are asked to identify the dye that will be visible after a gram-negative bacterium is stained using the Gram staining technique.
1. **Crystal violet:** This is a primary stain used in Gram staining. Although it initially stains all bacteria, it is not retained by gram-negative bacteria after the decolorization step.
2. **Safranin:** This is a counterstain applied after decolorization. It stains the gram-negative bacteria pink or red.
3. **Decolorizer:** This is usually an alcohol or acetone solution that removes the crystal violet stain from gram-negative bacteria.
4. **Iodine:** This is a mordant applied after the crystal violet. It forms a complex with crystal violet that helps retain the stain in gram-positive bacteria but not in gram-negative bacteria.
**Correct Answer:**
- [ ] Safranin](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F27937a9a-6c56-4023-8617-cb9097cc0b1e%2F3dc665d9-4af0-4994-b51e-6a13142e8d48%2Fybjuz2a_processed.png&w=3840&q=75)
Transcribed Image Text:### Gram Staining of Gram-Negative Bacteria
**Question:**
If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is completed?
**Options:**
- [ ] Crystal violet
- [ ] Safranin
- [ ] Decolorizer
- [ ] Iodine
In this multiple-choice question, students are asked to identify the dye that will be visible after a gram-negative bacterium is stained using the Gram staining technique.
1. **Crystal violet:** This is a primary stain used in Gram staining. Although it initially stains all bacteria, it is not retained by gram-negative bacteria after the decolorization step.
2. **Safranin:** This is a counterstain applied after decolorization. It stains the gram-negative bacteria pink or red.
3. **Decolorizer:** This is usually an alcohol or acetone solution that removes the crystal violet stain from gram-negative bacteria.
4. **Iodine:** This is a mordant applied after the crystal violet. It forms a complex with crystal violet that helps retain the stain in gram-positive bacteria but not in gram-negative bacteria.
**Correct Answer:**
- [ ] Safranin
Expert Solution

This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
This is a popular solution!
Trending now
This is a popular solution!
Step by step
Solved in 2 steps

Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Recommended textbooks for you

Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON

Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax

Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,

Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON

Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax

Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,

Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company

Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.

Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education