Decolorization in Gram-staining removes safranin from gram-negative cells True False
Q: How does cotton plug prevent contamination of the medium? [Answer in not more than 2 sentences
A: Cotton plugs are used in various experiments as lids to cover the mouth of the flask. It is usually…
Q: How is the India ink capsule stain an example of a negative stain?
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: hello, can you draw the following on endospore staining in 1000x objective? thank you. - 1 day…
A: Bacillus is a genus which is a endospore forming, rod shaped, gram positive bacteria.
Q: Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram…
A: Gram staining is the common procedure followed to distinguish the bacteria based on their cell wall.…
Q: Explain the significance of crystal violet, iodine, Grams decolorizer, and safrin make in Gram…
A: Gram staining is a common laboratory technique in microbiology that is used to differentiate…
Q: what color would you expect for a gram positive microorganism for each incorrect mistakes while…
A: "Gram staining" is a technique used in microbiology. It is a method in which a bacterium can be…
Q: When you interpret a Gram-stained smear, you should also describe the morphology (shape) of the…
A: Bacteria These are prokaryotic organisms which kack membrane bound organelles. Through the technique…
Q: Why is the Gram stain considered a differential stain?
A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…
Q: Describe the bacteria observed in different staining procedures. Please refer to the photo -…
A: Bacteria can be stained by a variety of stains so that they can be visualised in contrast to the the…
Q: Why is it sufficient to stain cells from a SDA (Sabouraud Dextrose Agar) plate with crystal violet…
A: Introduction : Sabouraud agar, often known as Sabouraud dextrose agar (SDA), is a peptone-containing…
Q: Of what value is Gram staining?
A: Staining the bacteria can be (a) Simple staining that reacts uniformly to all cell types and only…
Q: What color would gram positive cells appear if ghr safranin staining step was left out of staining…
A: Gram staining is a technique used for differentiating the bacteria based on their cell wall…
Q: Move the images of bacterial cells into the chart to illustrate the color of gram-positive and…
A: Gram staining is a technique used to differentiate between two groups of bacteria namely…
Q: Why is oil immersion necessary to see bacteria in the laboratory?
A: Oil immersion technique is used to increase the resolving power of microscope. Both the objective…
Q: Give the principle of Grams stain, Ziehl-Neelsen, and Kinyoun Staining methods
A: Ziehl neelsen staining principle The presence of mycolic acids in the cell walls of acid-fast…
Q: Why should a young culture be used in gram staining rather than an old culture? b. Why is it…
A: To answer this question first, we need to understand what is gram staining and what are the steps of…
Q: why are the steps in gram staining are so carefully standardized?
A: Gram staining is very useful process that differentiate bacteria on the basis of their cell wall…
Q: Match the expected result (purple, red, or colorless) to the following descriptions of Gram-stained…
A: Gram-staining is a procedure of staining than can be used to differentiate between bacterial…
Q: What would be the result of the gram stain if acid alcohol is used as a decolorizer rather than the…
A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…
Q: Gram staining is the most common initial test of unknown identification, explain in your own words…
A: The Gram staining is a typical process that is used to separate two groups of microorganisms based…
Q: Explain what happens to Gram-positive and Gram-negative bacterial cells at each step of the…
A: The process of Gram Staining on any sample requires four basic steps that include applying a primary…
Q: Gram-positive (+) bacteria with a thin peptidoglycan layer traps the crystal violet stain. Select…
A: Gram staining is also known as Gram's method is a staining method for identifying and separating…
Q: Explain the different results in the Gram stain are due to differences in the structure of the cell…
A: There are many types of bacteria and bacterial species. They are prokaryotic cell which has a cell…
Q: Give the stages of gram staining
A: Gram staining also termed Gram's method is defined as a staining method for distinguishing and…
Q: Give the procedure of Sabin-Feldman dye test. Describe the positive and negative results.
A: The Sabin Feldman dye test is used to diagnose the toxoplasmosis, which is a disease caused by…
Q: Which cellular structure is being detected in the motility test? bacterial ribosomes bacterial cilia…
A: Prokaryotes are characterised by the absence of nucleus and membrane bound organelles.
Q: This part of the staining process helps the primary stain to remain in the cell during…
A: Staining of biological samples is a very useful process for detailed observation and measurement of…
Q: Describe the bacteria observed in different staining procedures. Please refer to the photo - Direct…
A: The cell wall of most bacteria has an overall net negative charge and thus can be stained directly…
Q: what effect would failing to drain the smear in-between staining have on your judgment of the gram…
A: Gram staining is a technique used to differentiate between two groups of bacteria namely…
Q: hat stain technique must this be? What is used to stain the background? What is used to stain the…
A: Capsules have a low staining ability with conventional dyes. Typically, capsule staining procedures…
Q: What is the purpose of iodine in the gram staining process? Explain.
A: Gram stains are used to distinguish between bacteria with and without cell walls. When the gramme…
Q: How does smear preparation of cells from a liquid medium differ from preparation of cells from a…
A: Answer: Smear Preparation is the method in microbiology laboratories to place the small amount of…
Q: Select all that apply to a negative stain: 1. involves a washing step 2. cells may be distorted…
A: Acid staining is a type of negative staining. This indicates that the dye readily releases a proton…
Q: The GRAM stain method was applied on both pictures. What is the result found on each of them? A A.
A: The bacteriologist Hans Christian Gram was developed gram staining method in 1884. the Gram…
Q: What staining technique is used here? What is the primary stain, decolorizing agent, and counter…
A: Gram Staining is the common, important, and most used differential staining techniques in…
Q: Specific Dyes used in flagella stain: A wet-mount procedure (Ryu method) Dried-smear preparation…
A: Flagella is a hair-like structure that is found in bacteria, archaea, and Eukaryota. It allows the…
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- Question 6 Describe what the Gram Stain shows: a. The organism is Gram Negative b. The organism is this shape: cocci c. The organism has this arrangement: Question 7If you Gram stain a bacterium that is gram-negative, which dye will be visible once the stain is completed? Crystal violet Safranin Decolorizer lodineWhat is the purpose of each reagent in gram-staining? Primary stain Mordant Decolorizing agent Counterstain
- Give two reasons for the differential staining between gram + and gram - organismsIs is best to observe slides of bacteria stained with the acid fast stain under high dry magnification. True or falseExplain the relationship between bacterial cell wall with gram-positive and gram-negative in the staining process. Please state chemical used and their function.
- Contrast the overall cell wall compositions of Gram positive and Gram negative bacteria, and go step-by-step in the procedure of the Gram stain. Describe what is occurring during each step within both Gram-positive cells and in Gram-negative cells. Be sure to include the appearance of each cell type at the end of each step.Explain the roles of techoic acids in gram positive structure and in gram staining.Examples of bacteria observe in the microscope using Gram stain, Ziehl-Neelsen, and Kinyoun Method.
- Which of the following serves as a counterstain for the gram-stain technique? It also happens to be the counterstain for the endospore staining technique.An acid-fast stain can be used to detect protective coverings found in using light microscopy. organisms incorporating mycolic acid in the cell wall organisms forming endospores organisms with capsules organisms with Gram-negative cell wallsAdditional Differential Staining Procedures: Negative stain, Capsule Stain, Endospore stain, Flagella stain. What is a positive and negative results of this procedures?