If you have a mixed (Gram positive and Gram negative) sample, and you complete a Gram stain, and all the cells are purple at the end of the stain, what have you done wrong? forgotten safranin forgotten crystal violet forgotten decolorizer forgotten iodine everything worked perfectly

Q: Explain the reasons you would use a negative stain?

A: In a negative stain the slide is actually stained. This is because the negatively charged stain…

Q: Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram…

A: Gram staining is the common procedure followed to distinguish the bacteria based on their cell wall.…

Q: When preparing a "wet mount" slide of your own cheek cells, what is the name of the wet medium that…

A: Cheek cells are human cells and eukaryotic cells which can be visualized under the microscope. These…

Q: You look at a slide you prepared and, despite the fact that you know you put organism onto the slide…

A: Gram-staining is a laboratory test that detects the presence of bacteria and also helps to…

Q: Why is heat necessary in spore staining?

A: BASIC INFORMATION  SPORULATION  It is a process during which spores are formed.  We can find it in…

Q: what color would you expect for a gram positive microorganism for each incorrect mistakes while…

A: "Gram staining" is a technique used in microbiology. It is a method in which a bacterium can be…

Q: Crystal violet is an example of what type of stain?

A: A stain is used to clearly view a specimen under the microscope. For staining, a smear of the…

Q: Why must heat or a surface-active agent be used with application of the primary stain during…

A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…

Q: hich Is The More Important Step In Gram Stain?

A: Hans Christian Gram invented the Gram stain in 1884. (Gram,1884).

Q: Why is the Gram stain considered a differential stain?

A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…

Q: An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and…

A: Introduction: Acid-fast staining is a type of differential staining technique that was first…

Q: A capsule stain is kind of: A Simple and Negative Stain B Differential and negative stain C…

A: Introduction  Capsule is the outer most covering, which is present even outside the cell wall. This…

Q: Which of the following statements is FALSE?   Question options:   stains increase the contrast of…

A: 3rd statement is false i.e the chromophore of an acidic dye is positively charged.

Q: Bacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed…

A: Bacteria have cell walls made up of polysaccharides that give them strength.Bacterial cell walls…

Q: What color would gram positive cells appear if ghr safranin staining step was left out of staining…

A: Gram staining is a technique used for differentiating the bacteria based on their cell wall…

Q: Move the images of bacterial cells into the chart to illustrate the color of gram-positive and…

A: Gram staining is a technique used to differentiate between two groups of bacteria namely…

Q: In your own words, explain how gram staining is done and how it works.

A: Gram staining is a popular method for distinguishing between two large groups of bacteria based on…

Q: Acid-Fast stain M. Smegmatis -morphology shape -Arrangement -Acid-fast or non-acid-fast

A: Introduction :- Mycobacterium smegmatis is a rod-shaped bacterium that is commonly used as a model…

Q: Why is it sometimes difficult to attain both good resolution and good contrast at the same time for…

A: Magnification and resolution are important because the magnification in larges the image and the…

Q: When it comes to simple stains, why is time so important? And what is the purpose of rinsing the…

A: Microbiology is a hugely important field in both basic and applied research. New and fascinating…

Q: Select all that apply to a direct simple stain:    1. the dye is acidic or negatively charged and…

A: Gram staining is used to examine the shape of the bacterial cells; if they are contaminated.…

Q: Why should a young culture be used in gram staining rather than an old culture? b. Why is it…

A: To answer this question first, we need to understand what is gram staining and what are the steps of…

Q: -What are the steps of gram staining -Which of the four steps is the most important and why? - What…

A: Based on the chemical and physical characteristics of their cell walls, the Gram stain is an…

Q: Why is it important to perform a gram stain for any type of bacteria? After performing the gram…

A: The gram staining is a procedure that is useful in classifying the bacteria based on retention of…

Q: What are the advantages and disadvantages of gram staining

A: Introduction  Gram staining is a widely used laboratory technique in microbiology to differentiate…

Q: Gram E. coli 3nnell rod shaped 1000 x - around 1-2 micrometers long gative grom should be pink ich…

A: Gram Staining It is a differential bacterial staining techniques used to differentiate Bacteria…

Q: Create paragraphs to differentiate among the following terms: positive stain, negative stain, simple…

A: An organic molecule with a benzene ring, a chromophore, and an auxochrome group is known as a…

Q: When doing a gram stain in microbiology, one step can be eliminated and still allow distinction…

A: Please follow step 2 for detailed explanation.

Q: Gram staining is the most common initial test of unknown identification, explain in your own words…

A: The Gram staining is a typical process that is used to separate two groups of microorganisms based…

Q: What is the advantage of the Kinyoun staining procedure over the Ziehl-Neelsen method?

A: Kinyoun staining is a technique used to stain bacteria. Specifically, acid-fast species of the…

Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used

A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…

Q: After performing the gram stain you observe your result as gram negative. To help confirm this…

A: Gram staining is a technique used for staining the microbial samples. It was performed by the…

Q: Why is nigrosin-water- bacteria puddle need to be mixed in a Simple/Negative staining

A: Staining is a technique used in slide preparation to color the specimen. With light microscopy,…

Q: Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH of the stain…

A: 1.Since the bacteria are negatively charged, a positive charge was needed to cause staining. The…

Q: two different

A: Gram staining- A test that checks for bacteria at the site of a suspected infection such as the…

Q: List the  steps in a Gram stain.

A: Introduction: The principle of Gram's stain depends upon the Gram's reaction. The Gram's reaction…

Q: You and a partner conduct the acid-fast staining using the same two bacteria. You know that there…

A: Acid-fast staining is a differential staining technique that proves to be an important diagnostic…

Q: Explain the principle of Gram staining. (You do not need to describe the procedure step by step).

A: The danish Bacteriologist Christian gram discovered this differential technique in order to…

Q: Why don't you heatfix a negative stain slide?

A: Negative staining is used to investigate the physical structure, size, and organization of…

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.