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Basic Clinical Laboratory Techniques 6E

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.4: The Gram Stain

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**Educational Content: Ziehl-Neelsen Acid Fast Stain**

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**Question:**

What is the primary stain for the Ziehl-Neelsen acid fast stain? What color is this stain? What cells will display this stain once you finish the stain and view your slide?

**Answer:**

In the Ziehl-Neelsen acid fast stain procedure, the primary stain used is carbol fuchsin. This stain imparts a reddish-pink color to acid-fast bacteria. Cells that are not acid-fast will generally appear blue or green, depending on the counterstain used (commonly methylene blue or brilliant green). The acid-fast cells, which include bacteria such as Mycobacterium species, retain the red color of carbol fuchsin even after decolorization with an acid-alcohol solution.

By completing this staining procedure and examining your slide under a microscope, you should be able to identify and differentiate between acid-fast bacteria (which will appear reddish-pink) and non-acid-fast cells (which will appear blue or green).

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This explanation helps illustrate the use and outcome of the Ziehl-Neelsen acid fast stain in a microbiological context, aiding in the identification of specific bacterial types based on their staining properties.

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