What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What cells will display this stain once you finish the stain and view your slide?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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**Educational Content: Ziehl-Neelsen Acid Fast Stain**

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**Question:**

What is the primary stain for the Ziehl-Neelsen acid fast stain? What color is this stain? What cells will display this stain once you finish the stain and view your slide?

**Answer:**

In the Ziehl-Neelsen acid fast stain procedure, the primary stain used is carbol fuchsin. This stain imparts a reddish-pink color to acid-fast bacteria. Cells that are not acid-fast will generally appear blue or green, depending on the counterstain used (commonly methylene blue or brilliant green). The acid-fast cells, which include bacteria such as Mycobacterium species, retain the red color of carbol fuchsin even after decolorization with an acid-alcohol solution. 

By completing this staining procedure and examining your slide under a microscope, you should be able to identify and differentiate between acid-fast bacteria (which will appear reddish-pink) and non-acid-fast cells (which will appear blue or green).

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This explanation helps illustrate the use and outcome of the Ziehl-Neelsen acid fast stain in a microbiological context, aiding in the identification of specific bacterial types based on their staining properties.
Transcribed Image Text:**Educational Content: Ziehl-Neelsen Acid Fast Stain** --- **Question:** What is the primary stain for the Ziehl-Neelsen acid fast stain? What color is this stain? What cells will display this stain once you finish the stain and view your slide? **Answer:** In the Ziehl-Neelsen acid fast stain procedure, the primary stain used is carbol fuchsin. This stain imparts a reddish-pink color to acid-fast bacteria. Cells that are not acid-fast will generally appear blue or green, depending on the counterstain used (commonly methylene blue or brilliant green). The acid-fast cells, which include bacteria such as Mycobacterium species, retain the red color of carbol fuchsin even after decolorization with an acid-alcohol solution. By completing this staining procedure and examining your slide under a microscope, you should be able to identify and differentiate between acid-fast bacteria (which will appear reddish-pink) and non-acid-fast cells (which will appear blue or green). --- This explanation helps illustrate the use and outcome of the Ziehl-Neelsen acid fast stain in a microbiological context, aiding in the identification of specific bacterial types based on their staining properties.
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