During staining, the first step is to dewax the slide. What is the purpose of the step? What is the effect of insufficient dewaxing?

Q: (a) Which would be easier to prepare bacterial smear from, liquid or solid culture? Why? (b) Aside…

A: Introduction A bacterial smear is a small layer of bacteria that has been stained on a slide. A…

Q: Why must heat or a surface-active agent be used with application of the primary stain during…

A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…

Q: Why are basic dyes more effective for bacterial staining than acidic dyes?

A: Staining is a technique used in slide preparation to colour the specimen.      With light microscopy…

Q: An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and…

A: Introduction: Acid-fast staining is a type of differential staining technique that was first…

Q: Would phase microscopy be of any particular value when examining a stained specimen? Explain

A: The area or field of study in which microscopes are used to view an object that cannot be seen…

Q: Which of the following are true about a differential stain. There may be more than one answer!…

A: A microscopic organism made of one cell is referred to as a prokaryote. Prokaryotic organisms do…

Q: What is the difference between simple stain and complex stain? Describe the color of an S. aureus…

A: Most types of cells don't have a normal pigment and are in this way challenging to see under the…

Q: Which step is the most crucial or most likely to cause poor results in the Gram stain?

A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…

Q: Differentiate between negative and positive staining, givingexamples.

A: These are the staining methods that are used to see bacteria under a microscope.

Q: For a laboratory exercise of basic techniques in microbiology: Gram stain and Microscopy. What are…

A: "Gram staining is important in microbiology because it allows scientists to quickly and easily…

Q: Describe how simple staining and fluorescence staining are similar and how they are different. What…

A: Microscopy is a technical field for the use of microscopes to visualize objects and locations of…

Q: What is the advantage of the Kinyoun staining procedure over the Ziehl-Neelsen method?

A: Kinyoun staining is a technique used to stain bacteria. Specifically, acid-fast species of the…

Q: Can colorless samples be analyzed using a UV-Vis spectrophotometer? Yes or No or Maybe?

A: UV-Vis Spectroscopy  It is defined as a quantitative technique that is used to identify the…

Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used

A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…

Q: D) When performing fluorescence microscopy what are the stokes shift and why is it better  to have…

A: Answer: Fluorescence Microscopy  In fluorescent microscopy , the specimen we use is either…

Q: Why don't you heatfix a negative stain slide?

A: Negative staining is used to investigate the physical structure, size, and organization of…

Q: What is phase contrast? Give examples of phase contrast in optical microscopy

A: Microscopy is the technique for visualisation of ultra-small objects such as cells or even smaller…

Q: What is the advantage of cryo-sectioning for antibody staining methods?

A: To study the antigenic structures, present on the cell surface tissues can be embedded in paraffin…

Q: Why is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?

A: Microtomes are specialized cutting instruments that produce very thin slices of tissues so that the…

Q: What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary…

A: Need to explain the primary stain in the Kinyoun acid-fast stain.

Q: microscopy, why are different stains used for different samples? Be specific.

A: A microscope is an instrument which is used to observe microscopic specimens which are otherwise not…