Did your gram stain work? What about your endospore stain? How do you know/what things were you looking for to confirm you succeeded?Please help me ! GramE. colismall rod shaped1000 xaround 1-2 micrometerslongnegative gromshould be pinkwhich it was.Grome. Coli Gram +Basillys Subtiliswhich is 1623rad shapedcan Sonn endospore1000xaround 1-4 micrometersгодpositive gramshould be purpleGram +Bacillus Subtilis

Answered: Gram E. coli 3nnell rod shaped 1000 x -…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

Did your gram stain work? What about your endospore stain? How do you know/what things were you looking for to confirm you succeeded? Please help me !

Gram
E. coli
small rod shaped
1000 x
around 1-2 micrometers
long
negative grom
should be pink
which it was.
Grom
e. Coli

Gram +
Basillys Subtilis
which is 1623
rad shaped
can Sonn endospore
1000x
around 1-4 micrometers
год
positive gram
should be purple
Gram +
Bacillus Subtilis

Expert Solution

Step 1

Gram Staining

It is a differential bacterial staining techniques used to differentiate Bacteria into gram positive and gram negative types according to their cell wall compositions. It is generally the first test performed in Bacteria during the identification and observation process.

Objective

To differentiate between the gram positive and gram negative Bacteria
To study vtge morphological structure of bacteria.

The gram positive bacteria have a single plasma membrane with a thick superficial peptidoglycan cell wall. Gram negative Bacteria have the two cell walls and a thin peptidoglycan cell wall in between them .

Procedure

Flood crystal violet solution over the Fixed smear
After 30-60 seconds , pour off the crystal violet solution , and rinse with gentle running water
Flood the grams iodine solution over the smear
Leave the iodine solution for 30-60 seconds and pour of the excess iodine and rinse with gentle running water.
Shake off the excess water over the smear
Decolorize the smear by passing the decolorisimg solution . And rinse with distilled water after 5 seconds
Remove the excess water on the smear
Air dry the smear .

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