What is the best wavelength to study light scattering with bacteria?     260 nm     260-280 nm     450 nm     280 nm

Q: How is the India ink capsule stain an example of a negative stain?

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: Is it possible to perform double or triple staining using fluorescent dyes? Explain

A: For both membrane and soluble proteins, fluorescent dyes are increasingly being utilized to monitor…

Q: Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram…

A: Gram staining is the common procedure followed to distinguish the bacteria based on their cell wall.…

Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…

A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…

Q: During endoscope staining method we use the boiling of water on a tripod technique. WHY?

A: Certain Gram positive bacteria produce a tough, dormant life saving stage which is non reproductive,…

Q: Why is heat necessary in spore staining?

A: BASIC INFORMATION  SPORULATION  It is a process during which spores are formed.  We can find it in…

Q: What do you think is/are the characteristic of sulfamethoxazole (antibiotic) that allows it to…

A: This is the structure of Sulfamethoxazole.

Q: Why is the wavelength of the light that is released in spectrophotometric analysis longer than the…

A: Some molecules absorb light at a particular wavelength and emit light in higher wavelength and this…

Q: How can a UV-Vis spectrophotometer be applicable in determining the absorbance/concentration of a…

A: A spectrophotometer is an instrument that is used to measure the intensity of light absorbed by the…

Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…

A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…

Q: Describe how simple staining and fluorescence staining are similar and how they are different. What…

A: Microscopy is a technical field for the use of microscopes to visualize objects and locations of…

Q: What are the advantages and disadvantages of gram staining

A: Introduction  Gram staining is a widely used laboratory technique in microbiology to differentiate…

Q: What are the limitations of UV Visible spectroscopy and fluorescence spectroscopy? Include reference…

A: Spectrophotometric analysis has been widely used in biomedical science to determine the…

Q: What is FRAP (Fluorescence Recovery After Photobleaching)? Draw a graph of fluorescence versus time…

A: FRAP or Fluorescence Recovery After Photobleaching It is a method that was developed in 1976 by…

Q: Can colorless samples be analyzed using a UV-Vis spectrophotometer? Yes or No or Maybe?

A: UV-Vis Spectroscopy  It is defined as a quantitative technique that is used to identify the…

Q: list 4 advantages and 4 limitations of the Gram staining process

A: Gram staining process is the procedure which is uses differential gram stain which has two dyes to…

Q: Explain the difference in the working principles of Dark-field and Light-field microscopes using pKk…

A: Microscope: It is an optical instrument that uses a lens or a combination of lenses to produce…

Q: Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH of the stain…

A: 1.Since the bacteria are negatively charged, a positive charge was needed to cause staining. The…

Q: What is the main advantage of fluorescence polarization? Sensitivity of fluorescent probes Low cost…

A: FP is an analytical technique which provides information on orientation and mobility of…

Q: Discuss the concept of spectrophotometry, colorimetery, and turbidimetry. Maximum of 150 words

A: Spectrophotometry is a part of electromagnetic spectroscopy worried about the quantitative…

Q: What is safe view dye for? Compare it with Ethidium Bromide.

A: Safe view dye is a nucleic acid stain which is used for the detection of double-stranded DNA,…

Q: What is the difference between Giemsa and Wright stain?

A: Staining is a technique used to enhance contrast in samples, generally at the microscopic level.…

Q: Briefly explain the difference between Wright Staining and H&E Staining (why use one over the other)

A: Staining is defined as a technique ,which is utilized for increasing the contrast in biological…

Q: Why is it called as differential stain

A: Staining is a technique used in slide preparation to color the specimen.With light microscopy,…

Q: The above photograph (IMAGE ATTACHED) of a cheek smear was obtained using the high-power objective…

A: The eyepiece graticule is a scale that is placed in the eyepiece of a microscope containing scales…

Q: What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What…

A: In microbiology, the technique that is used to enhance the contrast in samples, at the microscopic…

Q: What is the difference between Raman Spectroscopy and SERS ( Surface Enhanced Raman Spectroscopy).

A: Introduction: It is the most powerful tool to study the interactions between matter and…

Q: What is acid fast staining and why do we use gram staining more?

A: Staining is a method in which images of specimens enhanced at microscopic level. It is commonly used…

Q: Why do you have to reduce the amount of light with the diaphragm in order to see bacteria in a…

A: Microscope diaphragm  Diaphragm or iris of microscope is found at the base of the microscope whose…

Bacterial Morphology

The bacteria are prokaryotic organisms that are single-celled, and are found to exist as free-living and possess a microscopic size. The morphology is found to vary in the bacteria, where some of them are identified as individual organisms and the others are detected as colonies. The size and shape of the bacterial cell also represent its morphology. 

Bacterial cell structure

Bacteria are single-celled, tiny creatures that may enter healthy tissues and grow rapidly. Bacteria are microscopic organisms that are tiny and unicellular. These are members of the prokaryote kingdom. They live in water, air, soil, and all-natural environments. They are used in industrial and therapeutic processes, and they support a wide range of plant and animal life. The first organism to appear on the planet. Bacteria-like creatures are the oldest known fossils. Bacteria can consume a wide range of organic and inorganic elements, and some may even survive in harsh conditions.