Briefly explain the difference between Wright Staining and H&E Staining (why use one over the other)

Q: write the  protocol for Hexamine silver staining, including their reagents, materials, prinicple,…

A: Grocott’s alkaline hexamine-silver solution undergoes reduction to create precipitates of silver…

Q: The basic component of a fluoroscopic x-ray system suitable for interventional procedures consist of…

A: Radiography is a well-known imaging technique that uses X-rays, gamma rays, or other ionizing and…

Q: What are the main instrumentation components difference between Uv-visible and Fluorescence…

A: Spectroscopy :-  Spectroscopy is basically an association of electromagnetic radiation with atoms ,…

Q: Explain the mechanism of staining for each of the stains used. Iodine Methyl green Nigrosin

A: The living cells can be seen under a microscope. The cells is not visible properly as it is…

Q: Which of the following is true regarding aceto-orcein staining?

A: Aceto-orcein dye is a brownish-red and crystalline dye. It consists of a variety of phenaxazones,…

Q: OHOW Does usin stain chanGre The visibility a et The structures ? why?

A: Introduction Stains are dyes that are frequently used in biology and medicine to highlight…

Q: List other fluorescent stains. What is the usefulness of each?

A: Fluorescent stains/dyes are non-protein molecules that are employed in visualizing samples under a…

Q: a. Rank the three cell membranes in terms of decreasing fluidity. Provide an explanation of your…

A: The plasma membrane is the outmost layer that holds the inner constituents of the cell and prevents…

Q: Know about the different staining techniques (simple, direct, negative, and differential), what is…

A: Staining techniques can provide valuable information about the morphology, structure, and…

Q: What are other staining methods that can be used for PAGE?

A: Introduction: PAGE electrophoresis is a subtype of gel electrophoresis whereby normal gel is…

Q: Choose the 4 most correct steps from the list below for a simple positive stain and organize them…

A: The above question is related to the laboratory technique of bacterial staining. Staining is a…

Q: Defi ne dyes and describe the basic chemistry behind the process of staining.

A: Staining is a method by which contrast in a sample is enhanced usually at the microscopic level.…

Q: What are the principle and basic concepts of NEGATIVE staining?

A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…

Q: Å Q/ Why don't we fixation to simple negative staining?

A: It is method followed to help view a specimen under the microscope to study its morphological…

Q: What are the limitations of UV Visible spectroscopy and fluorescence spectroscopy? Include reference…

A: Spectrophotometric analysis has been widely used in biomedical science to determine the…

Q: Create paragraphs to differentiate among the following terms: positive stain, negative stain, simple…

A: An organic molecule with a benzene ring, a chromophore, and an auxochrome group is known as a…

Q: Why is it important that the agarose is exposed to ethidium bromide, which is either added to the…

A: Ethidium bromide is utilized during separation of fragments of DNA by agarose gel electrophoresis.…

Q: Types of Differential Staining techniques.  1. Gram stain 2. Acid-fast stain     Please…

A: Staining is used in microscopy to highlight the structures to be viewed under microscope. It is of…

Q: Discuss sabin feldman dye test.

A: A Sabin–Feldman dye test is a serologic test for toxoplasmosis diagnosis. Toxoplasma trophozoites…

Q: A concept map for staining technique.

A:

Q: Explain the principle of the isoelectric focusing electrophoresis method and what it is used for.

A: Proteins are defined as the biological molecule that are produced by the building blocks amino…

Q: What are the principle and basic concepts of Simple staining

A: The simple staining doesn't give a lot of data about the cell separated from the bacteria'…

Q: Fluorescent stains can be viewed on ____________ microscopes.

A: On fluorescence microscopes, fluorescent stains can be observed. Fluorescent dyes or stains are used…

Q: write the  protocol for mann's methyl blue-eosin staining, including their reagents, materials,…

A: Reagents: Alkaline alcohol reagent –100ml of absolute Ethanol or Methanol containing 0.2ml 0.1N…

Q: Give a specific example on how to use UV-Vis spectrophotometer in medtech-medical field.  Provide a…

A: UV-Vis spectroscopy It is also called absorbance spectroscopy as it allows the analysis of the…

Q: What is safe view dye for? Compare it with Ethidium Bromide.

A: Safe view dye is a nucleic acid stain which is used for the detection of double-stranded DNA,…

Q: Define the following terms: a.Simple staining b.Complex medium c.Defined (synthetic) medium

A: Staining is used to visualise the bacteria i clinical specimen under a light microscope. Bacterias…

Q: Differentiate the “hot method” and “cold method” of acid-fast staining

A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…

Q: What is the difference between Giemsa and Wright stain?

A: Staining is a technique used to enhance contrast in samples, generally at the microscopic level.…

Q: Give the procedure of Sabin-Feldman dye test. Describe the positive and negative results.

A: The Sabin Feldman dye test is used to diagnose the toxoplasmosis, which is a disease caused by…

Q: Name a few group of microorganisms where Grams staining will not work and/or not appropriate to use.…

A: In Gram staining crystal violet (primary stain). stain is used to color the bacterial cell wall. The…

Q: Degree of clarity is known as contrast magnification resolution O wavelength

A: A microscope is a laboratory instrument used to examine objects that are too small to be seen by the…

Q: Briefly answer the question below: What is the disadvantage of having a really thick smear when…

A: Smear:- It is used to fix the bacteria onto the slide and to prevent the sample from being lost…

Q: list the stains and decolorizing agent used in acid fast staining method.

A: Certain bacteria have a physical property known as acid fastness. Property:- Resistance to…

Q: What is a crystal violet stain and its purpose?

A: Crystal violet is that the name given to the cluster of similar organic compounds that are used as…

Q: Discuss the steps from dissection of tissue to producing a section on a slide ready for staining, as…

A: Human tissue has long been recognized for its extraordinary value in medical research. These cells…

Q: Can someone explain the concept behind a sample that lies in the UV range in a spectrophotometer?…

A: UV spectroscopy or UV–visible spectrophotometry refers to absorption spectroscopy or reflectance…

Q: In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used…

A: UV/Visible Spectrophotometry detects the absorbance of light in the UV/Visible range of the…

Q: Adjusting the objective aperture is an important step to obtain phase contrast in TEM. Compare the…

A: The "objective aperture" is used for accepting a transmitted wave or one of diffracted waves to…

Q: Write the protocol for lactophenol blue stain method indicating; materials,  reagents,  Principle,…

A: Fungi consist of a broad class of organisms from single yeast to multicellular organisms like…