Three cell membrane samples: M, C, and S were fluorescently labeled and were subsequently photobleached. The recovery of fluorescence was monitored and gave the following profile: 100 M C time a. Rank the three cell membranes in terms of decreasing fluidity. Provide an explanation of your ranking based on their FRAP curves and the most probable appearance and composition of the three membranes. b. Draw the expected image of the surface of the three membranes before photobleaching and after fluorescence recovery as viewed under a fluorescence microscope. % fluorescence
Three cell membrane samples: M, C, and S were fluorescently labeled and were subsequently photobleached. The recovery of fluorescence was monitored and gave the following profile: 100 M C time a. Rank the three cell membranes in terms of decreasing fluidity. Provide an explanation of your ranking based on their FRAP curves and the most probable appearance and composition of the three membranes. b. Draw the expected image of the surface of the three membranes before photobleaching and after fluorescence recovery as viewed under a fluorescence microscope. % fluorescence
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Please answer both questions A and B. Question b needs drawing so clearly draw what is given. An illustration (IMAGE 2) can be use as guide
Transcribed Image Text:Three cell membrane samples: M, C, and S were fluorescently labeled and were subsequently
photobleached. The recovery of fluorescence was monitored and gave the following profile:
100
M
S
time
a. Rank the three cell membranes in terms of decreasing fluidity. Provide an explanation of
your ranking based on their FRAP curves and the most probable appearance and composition
of the three membranes.
b. Draw the expected image of the surface of the three membranes before photobleaching
and after fluorescence recovery as viewed under a fluorescence microscope.
% fluorescence
Transcribed Image Text:FRAP (Fluorescence Recovery After Photobleaching)
(A) 1
Immobile fraction
Photoech
2
3
Recovery
half time
Time
Bleach
6
Fluorescence intensity in
bleach ROI
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