**Main Advantage of Fluorescence Polarization:**1. **Sensitivity of fluorescent probes** (highlighted in yellow)2. Low cost of instrument and reagents3. Compatibility of reagents with most analytes4. **No need to purify samples** (highlighted in red)5. Easy to attach fluorescent probe

FP is an analytical technique which provides information on orientation and mobility of…

Answered: What is the main advantage of…

Principles Of Radiographic Imaging: An Art And A Science

6th Edition

ISBN:9781337711067

Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Chapter23: Technical Considerations In Digital Imaging

Section: Chapter Questions

Problem 11RQ

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3) Define gel electrophoresis, including its theory and application. Describe the steps of running gel electrophoresis using the following image. More detailed reading: https://www.sciencedirect.com/topics/medicine-and-dentistry/agar-gel-electrophoresis POWER SUPPLY CATHODE ELECTROPHORETIC BUFFER ANODE WELL SAMPLE AGAROSE GEL POWER SUPPLY CATHODE ANOCE HIGH MOLECULAR WEIGHT SPECIES LOW MOLECULAR WEIGHT ANALYTES
How could you utilize fluorescence quenching in the different analytical application? Please answer shorty at your own words. Answer should be to the point.
In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used in the mixture, the two proportions should be totalled to 1.0. but on finding You got 0.6 in total. Explain the reasons for the difference.
Select all that apply to a direct simple stain: 1. the dye is acidic or negatively charged and stains the background 2. uses multiple dyes in the procedure 3. cells will never be distorted or shrunken 4. uses only 1 dye in the procedure 5. does involve a washing step 6. can be used to determine cell morphology, size, and arrangement. 7. does involve fixing 8. the dye is basic or positively charged and stains the bacteria 9. cells may be distorted or shrunken 10. does not involve a washing step 11. does not involve fixing
Agarose Gel Electrophoresis Questions: 1. What determines how much agarose you should use in your gel? 2. Why do you see only DNA on the gel, and not protein? 3. How do you know which end of the gel to place the comb? 4. Suppose you turn on your power supply to run the gel and find that the milliamps reading is close to zero. What would you check?
Why is UV light used in Column chromatography experiment ?
To determine whether proteins interact with each other which two techniques might you use 1. Yeast 2 hybrid system and western blotting 2. polymerase chain reaction and gel filtration chromatography 3. Yeast 2 hybrid system and affinity chromatography 4. yeast 2 hybrid system and gel filtration chromatography 5. light microscopy and yeast 2 hybrid system
Image 1 shows raw data of gel electrophoresis. Label/annotate image 1 (lanes, ladder sizes, etc). Explain what your seeing in the gel. Use the following information and picture 2 to assist in labelling. The purpose of this gel electrophorsis is to ensure that your GOI, FAP257, is in each BAC. Protocol that was done for Gel electrophoresis: -Place tray with gel into gel box -Fill gel box with IX TAE until the gel is completely submerged -Remove the comb for wells -Load 10ul of the 1kb Gene Ruler ladder (well 1) -Contains DNA ladder, 6X TriTrack DNA, Loading Dye, and Deionized water -Load other wells -Well 2: Control -Well 3: BAC- 15M5 -Well 4: BAC-39K10 -Well 5: BAC-27N17 -Run the gel at 100V for 30 minutes or until the dye front has migrated 2/3 down the gel
A fluorescence recovery after photobleaching (FRAP) was performed at 37 °C. If the experiment were carried out at 10 °C, what effect would you expect on the rate of fluorescence recovery, Why?
Why is the wavelength of the light that is released in spectrophotometric analysis longer than the wavelength of the light that is used to activate a fluorescent molecule?
name 5 things about method staining.

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