Hydrodynamic focusing is used in automated cell-counting instruments to: Prevent coincidence passage through the detection aperture Increase the visibility of the cells passing through the aperture Allow for maximal light absorbance through the cells passing through the aperture Ensure adequate uptake of fluorescent staining by the cells

Q: Density gradient centrifugation is an inexpensive cell separation technique. Mention 2 limitations…

A: Density gradient centrifugation is a common technique for isolating and purifying biomolecules and…

Q: Define fluorescent staining.

A: The advent of microscopy started with the development of optical microscopy. Then the level of…

Q: A student was observing a filament of blue green bacteria under low power (60X). The diameter of the…

A: A. Average length of one cell = 2.4 mm/ 40=0.06 mm Under 60X = 40 cells, each is 0.06 mm in length

Q: What are the main instrumentation components difference between Uv-visible and Fluorescence…

A: Spectroscopy :-  Spectroscopy is basically an association of electromagnetic radiation with atoms ,…

Q: Write the steps involved in simple staining ? And what the purpose of doing it ?

A: It is a conventional staining technique. The method only uses a single stain and is a direct…

Q: Given: The used ocular objective while taking this image, has magnifying power of 6x The used…

A: Given : Stage micrometer used has graduation = 0.01mm Ten graduation on ocular micrometer coincides…

Q: This is integral to obtaining the experimental data that lead to the development of this model…

A: Introduction ODE MODEL:- Ordinary differential equations or ODE models are used to model biological…

Q: An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and…

A: Introduction: Acid-fast staining is a type of differential staining technique that was first…

Q: The benefits of the negative stain include:  Mark all that apply:   1. No shrinkage or distortion…

A: The negative stain is a process that uses an acidic stain to identify cells from a medium. In…

Q: Please discuss why staining is important in visualizing microbes under the microscope. What…

A: Staining is an essential step in visualising microbes under the microscope. THE NECESSITY OF…

Q: Know about the different staining techniques (simple, direct, negative, and differential), what is…

A: Staining techniques can provide valuable information about the morphology, structure, and…

Q: Draw and label 3 cell types at low and high power. State the magnification for each one

A: Microscopes enable us to magnify specimens. In simple terms , magnification is an increase in size…

Q: What is the best wavelength to study light scattering with bacteria?     260 nm     260-280 nm…

A: 260-280 nm The best wavelength to study light scattering with bacteria depends on the specific…

Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…

A: Simple staining: Simple stain uses only one stain for staining bacterial cells. It gives basic…

Q: What stain would you use to differentiate between bean and ant cells? Explain and include your…

A: Staining is a procedure that involves the application of colored chemicals called dyes to specimens.…

Q: Dilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of the old culture…

A: Introduction Dilution is the process of lowering the concentration of a solute in a solution by…

Q: What is the biological benefit(s) to the organism to have detachment induced cell death?

A: Anoikis is the programmed cell death that occurs in cells that detach from the surrounding…

Q: Compare and contrast procedures for preparing and observing live and fixed stained cells.

A: Preparing and observing live and fixed stained cells are two common procedures in cell biology that…

Q: What step normally associated with staining bacterial cells is omitted when the dimensions of cells…

A: Step 1 Gram staining is a technique of staining that is used to differentiate and categorize…

Q: list 4 advantages and 4 limitations of the Gram staining process

A: Gram staining process is the procedure which is uses differential gram stain which has two dyes to…

Q: You may want to use this resource for this problem. If you do, submit the output along with your…

A: The confocal microscope is an advanced imaging technique that allows for high-resolution and…

Q: staining

A: Staining-  It is used to enhance contrast in samples, generally at the microscopic level. In other…

Q: Explicitly discuss the importance of staining in cytology

A: Stains are dyes which impart different colours to different cellular structure and chemicals in the…

Q: Briefly describe how magnetic bead microrheometry is used to measure the mechanical properties of…

A: A magnetic bead microrheometry  has been designed which allows the generation of forces up to 4.5…

Q: Based from the results obtained from Gram staining, explain why bacterial cells retained or changed…

A: Staining is a technique that is used to enhance the contrast of the microscopic image through the…

Q: Stains are used because O they bind evenly to all str they reduce contrast and make it easier to see…

A: Staining is a procedure in microscopy where a specimen to be viewed under the microscope is…

Q: Your first task in the lab is to learn how to culture these cells and begin expanding the culture…

A: Answer:  Yes, the cell culture media need to be changed more often than the other. The open culture…

Q: What is the highest magnification of the following microscopes? Bright field Dark field…

A: A microscope is an instrument that is used to view objects that are too small to be seen by the…

Q: The cell is usually placed after the monochromator, except in the case of using a multichannel…

A: A multi-channel analyzer is an instrument used in laboratory and field applications so to analyze an…

Q: In this problem, you will design a filter system for a flow cytometer. The four fluorescent dyes are…

A: The order in which the filters are arranged with respect to the unfiltered signal is determined by…

Q: You wish to centrifuge and pellet yeast cells using a centrifuge. The protocol says that you need to…

A: Centrifugation is the process by which particles in a solution are separated based on the density…

Q: laboratory? explain with diagram

A: The tissue culture is the general term for the removal of the cells, tissues or organs from an…

Q: Cellular stains are commonly used in bacteriology. Which of the following is NOT a reason to stain a…

A: Introduction :- Cell staining is a method that can be applied to improve microscopic views of cells…

Q: This part of the staining process helps the primary stain to remain in the cell during…

A: Staining of biological samples is a very useful process for detailed observation and measurement of…

Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…

A: The staining is done to enhance the visualization of cell and certain cellular component under a…

Q: Contrast the overall cell wall compositions of Gram positive and Gram negative bacteria, and go…

A: The bacteria are classified according to their cell wall composition into Gram positive and Gram…

Q: After performing the gram stain you observe your result as gram negative. To help confirm this…

A: Gram staining is a technique to identify bacterial stain gram-positive or gram-negative. It can be…

Q: You have a patient sample and have done a Gram Stain. When you look on the microscope, this is what…

A: After a clinical sample is received in the laboratory, the first step often is to do a mandatory…

Q: Define dyes and describe the basic chemistry behind the processof staining.

A: Detection and observation of cells and microorganisms in their natural state under microscope is…