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See the follow up procedure for determining colony counts and express the number of colony forming CEU) in scientific notation. Record "TNTC" for plates with colonies that are too numerous to count.

See the follow up procedure for determining colony counts and express the number of colony forming CEU) in scientific notation. Record "TNTC" for plates with colonies that are too numerous to count.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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### Analyzing Bacterial Growth on Agar Plates In this section, we will explore the bacterial growth observed on several agar plates as documented in the provided image. The image features four petri dishes, each labeled with different identifiers and dilutions. The bacterial colonies visible across the plates demonstrate various patterns and densities of growth. #### Petri Dish Descriptions 1. **Top Left Plate (Labeled "S", 10^-7)** - **Description**: This plate shows minimal bacterial growth, with a few scattered colonies visible. The sparse distribution of colonies suggests a high dilution factor. 2. **Top Right Plate (Labeled "S", 10^-7)** - **Description**: Similar to the top left plate, this dish also exhibits sparse colony formation, indicating high dilution. The colonies present are scattered across the surface. 3. **Bottom Left Plate (Labeled "R", 10^-6)** - **Description**: This plate has a higher density of bacterial colonies compared to the previous two plates. Several colonies are present, suggesting a lower dilution factor. Colony formation appears to be more clustered. 4. **Bottom Right Plate (Labeled "P", 10^-6)** - **Description**: This plate exhibits bacterial growth with noticeable characteristics. Along with numerous small colonies, there is a significant larger colony with a distinctive red center, likely representing a different bacterial strain or a mutation. #### Observations and Analysis - **Dilution Factors**: The top two plates with 10^-7 dilution factors show significantly fewer colonies compared to the bottom plates with a 10^-6 dilution factor. This indicates that the amount of bacteria present in the initial sample was significantly reduced by this dilution. - **Colony Morphology**: The morphology of the colonies can provide insights into the types of bacteria present. For example, the distinctive red-centered colony on the bottom right plate may indicate a different species or a unique mutation that differentiates it from the other colonies. #### Educational Insights This experiment provides a clear visual demonstration of how dilution affects bacterial colony formation. By comparing different dilution factors, students can understand the relationship between the concentration of bacterial samples and the resultant colony growth. Observing the variety of colony morphologies further enriches the learning experience by highlighting bacterial diversity. In microbiology, such practices are fundamental in estimating bacterial concentrations in samples and understanding the growth characteristics of different bacterial strains.
Transcribed Image Text:### Analyzing Bacterial Growth on Agar Plates In this section, we will explore the bacterial growth observed on several agar plates as documented in the provided image. The image features four petri dishes, each labeled with different identifiers and dilutions. The bacterial colonies visible across the plates demonstrate various patterns and densities of growth. #### Petri Dish Descriptions 1. **Top Left Plate (Labeled "S", 10^-7)** - **Description**: This plate shows minimal bacterial growth, with a few scattered colonies visible. The sparse distribution of colonies suggests a high dilution factor. 2. **Top Right Plate (Labeled "S", 10^-7)** - **Description**: Similar to the top left plate, this dish also exhibits sparse colony formation, indicating high dilution. The colonies present are scattered across the surface. 3. **Bottom Left Plate (Labeled "R", 10^-6)** - **Description**: This plate has a higher density of bacterial colonies compared to the previous two plates. Several colonies are present, suggesting a lower dilution factor. Colony formation appears to be more clustered. 4. **Bottom Right Plate (Labeled "P", 10^-6)** - **Description**: This plate exhibits bacterial growth with noticeable characteristics. Along with numerous small colonies, there is a significant larger colony with a distinctive red center, likely representing a different bacterial strain or a mutation. #### Observations and Analysis - **Dilution Factors**: The top two plates with 10^-7 dilution factors show significantly fewer colonies compared to the bottom plates with a 10^-6 dilution factor. This indicates that the amount of bacteria present in the initial sample was significantly reduced by this dilution. - **Colony Morphology**: The morphology of the colonies can provide insights into the types of bacteria present. For example, the distinctive red-centered colony on the bottom right plate may indicate a different species or a unique mutation that differentiates it from the other colonies. #### Educational Insights This experiment provides a clear visual demonstration of how dilution affects bacterial colony formation. By comparing different dilution factors, students can understand the relationship between the concentration of bacterial samples and the resultant colony growth. Observing the variety of colony morphologies further enriches the learning experience by highlighting bacterial diversity. In microbiology, such practices are fundamental in estimating bacterial concentrations in samples and understanding the growth characteristics of different bacterial strains.
--- **Spread Plate** Follow the procedure below to determine colony counts and express the number of colony-forming units (CFU) in scientific notation. If plates contain colonies that are too numerous to count, record "TNTC." | DILUTION | COLONY COUNT | CFU/mL - Use scientific notation | |------------|----------------|--------------------------------------------| | 1:10^7 | | | | 1:10^8 | | | ---
Transcribed Image Text:--- **Spread Plate** Follow the procedure below to determine colony counts and express the number of colony-forming units (CFU) in scientific notation. If plates contain colonies that are too numerous to count, record "TNTC." | DILUTION | COLONY COUNT | CFU/mL - Use scientific notation | |------------|----------------|--------------------------------------------| | 1:10^7 | | | | 1:10^8 | | | ---
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