write a short dicsussion based on the graph.. ProcedureAAdd50ofTop up to 50ml.LB-Mix5mlTake3mlmixtureto Add freshampicillintotheovernight culturewith 45 ml LBmixtureanalysethebothOD600brothThe mixture isthenWithdraw 3ml mixture toOvernight cultureMeasure every 15analyse (OD600)record aszero time-point.incubatedshakedincubator at 37°c 200rpmatmins. Stop until75 mins.(E-coli + LB borth)Mixovernight culture5mlTake3mlNo ampicilinmixturetoNo ampicilinB (control) with 45 ml LBbrothanalysetheOD600ThemixtureisthenWithdraw 3ml mixture toMeasure every 15mins. Stop until75 mins.shaked -analyse (OD600)record aszero time-point.incubatedatincubator at 37°c 200rpmGraph of Absorbance vs Time(mins)0.4500.4000.3620.3500.3000.2500.200 T0.1500.1000.0500.000-101020304050607080Time(mins)Figure LAbsorntion septum of e-coli in LB broth with ampicillin for Group 1, Group 2 and Group3Absorbance((600nm)

Answered: Procedure A Add 50 of Top up to 50ml.…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

write a short dicsussion based on the graph..

Procedure
A
Add
50
of
Top up to 50ml.
LB-
Mix
5ml
Take
3
ml
mixture
to Add fresh
ampicillin
to
the
overnight culture
with 45 ml LB
mixture
analyse
the
both
OD600
broth
The mixture is
then
Withdraw 3ml mixture to
Overnight culture
Measure every 15
analyse (OD600)record as
zero time-point.
incubated
shaked
incubator at 37°c 200rpm
at
mins. Stop until
75 mins.
(E-coli + LB borth)
Mix
overnight culture
5ml
Take
3
ml
No ampicilin
mixture
to
No ampicilin
B (control) with 45 ml LB
broth
analyse
the
OD600
The
mixture
is
then
Withdraw 3ml mixture to
Measure every 15
mins. Stop until
75 mins.
shaked -
analyse (OD600)record as
zero time-point.
incubated
at
incubator at 37°c 200rpm
Graph of Absorbance vs Time(mins)
0.450
0.400
0.362
0.350
0.300
0.250
0.200 T
0.150
0.100
0.050
0.000
-10
10
20
30
40
50
60
70
80
Time(mins)
Figure LAbsorntion septum of e-coli in LB broth with ampicillin for Group 1, Group 2 and Group
3
Absorbance((600nm)

Expert Solution

Step 1

In order to write the paragraph, it is important to understand what an absorption spectra means.

For the counting of bacterial population, two methods are used: viable and plate count methods, and spectrophotometric (turbidimetric) analysis. Through spectrophotometers, the total cell biomass can be estimated, both alive and dead. As a result of this technique, turbidity (cloudiness in a suspension) is calculated to detect bacterial population in a broth culture. Turbidity is directly proportional to cells in a culture, so an increase in turbidity indicates bacterial growth. By measuring light transmission through a sample, spectrophotometers can quantify turbidity. The amount of light transmitted decreases as the cell population increases.
A reading of absorbance or optical density indicates indirectly how many bacteria are present. On a graph, bacterial growth is plotted using absorbance, a logarithmic value.

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