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What is the dilution factor in the task given if the total viable cells is 140 [28×5(squares)] and the total nonviable cells is 50 [10x5(squares)]?

What is the dilution factor in the task given if the total viable cells is 140 [28×5(squares)] and the total nonviable cells is 50 [10x5(squares)]?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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What is the dilution factor in the task given if the total viable cells is 140 [28×5(squares)] and the total nonviable cells is 50 [10x5(squares)]?

TASK 1. From a 100-mL algal culture, 100-μL was mixed with 900-μL dilution buffer. From this dilution, 10-μL was mixed with 10-μL of Trypan Blue, and was transferred to a hemocytometer counting chamber. Following the same lab rules in the video, determine the percent cell viability if all 5 squares had the same count as the square below (show your solution): 8 2. Determine the concentration of the original 100-mL algal culture in cells/mL (show your solution).
Transcribed Image Text:TASK 1. From a 100-mL algal culture, 100-μL was mixed with 900-μL dilution buffer. From this dilution, 10-μL was mixed with 10-μL of Trypan Blue, and was transferred to a hemocytometer counting chamber. Following the same lab rules in the video, determine the percent cell viability if all 5 squares had the same count as the square below (show your solution): 8 2. Determine the concentration of the original 100-mL algal culture in cells/mL (show your solution).
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