What is the dilution factor in the task given if the total viable cells is 140 [28×5(squares)] and the total nonviable cells is 50 [10x5(squares)]? TASK1. From a 100-mL algal culture, 100-μL was mixed with 900-μL dilution buffer. From thisdilution, 10-μL was mixed with 10-μL of Trypan Blue, and was transferred to a hemocytometercounting chamber. Following the same lab rules in the video, determine the percent cellviability if all 5 squares had the same count as the square below (show your solution):82. Determine the concentration of the original 100-mL algal culture in cells/mL (show yoursolution).

The dilution factor is a measure of how much a solution has been diluted or concentrated. It is…

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What is the dilution factor in the task given if the total viable cells is 140 [28×5(squares)] and the total nonviable cells is 50 [10x5(squares)]?

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

What is the dilution factor in the task given if the total viable cells is 140 [28×5(squares)] and the total nonviable cells is 50 [10x5(squares)]?

TASK
1. From a 100-mL algal culture, 100-μL was mixed with 900-μL dilution buffer. From this
dilution, 10-μL was mixed with 10-μL of Trypan Blue, and was transferred to a hemocytometer
counting chamber. Following the same lab rules in the video, determine the percent cell
viability if all 5 squares had the same count as the square below (show your solution):
8
2. Determine the concentration of the original 100-mL algal culture in cells/mL (show your
solution).

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