You take 10 ml of a stock solution, which is at a concentration of 1000 phage/ml, and dilute it to a total of 100 ml. From the resulting solution you take 5 ml and dilute it to 25 ml, and from the latter you take 5 ml and make a total of 20 ml. a) It will be possible to know how many bacteriophage particles there will be in 1 ml of the last solution b) What is the dilution factor in each step, in the same order in which the dilutions are made? c) What is the total serial dilution factor?
You take 10 ml of a stock solution, which is at a concentration of 1000 phage/ml, and dilute it to a total of 100 ml. From the resulting solution you take 5 ml and dilute it to 25 ml, and from the latter you take 5 ml and make a total of 20 ml.
a) It will be possible to know how many bacteriophage particles there will be in 1 ml of the last solution
b) What is the dilution factor in each step, in the same order in which the dilutions are made?
c) What is the total serial dilution factor?
A serial dilution is a laboratory technique that uses a series of consecutive dilution steps to gradually reduce the concentration of a substance or microorganisms (e.g., cells, bacteria, viruses) in a solution. This method is commonly used in biological research to obtain samples or solutions with lower concentrations for various purposes, particularly in microbiology and molecular biology.
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