a) did the positive and negative controls work correctly in this ELISA? Explain. B) in the sample wells( wells3-7) some are darker blue than others. Explain what happened here and how you'd interpret the results?
a) did the positive and negative controls work correctly in this ELISA? Explain. B) in the sample wells( wells3-7) some are darker blue than others. Explain what happened here and how you'd interpret the results?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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a) did the positive and negative controls work correctly in this ELISA? Explain.
B) in the sample wells( wells3-7) some are darker blue than others. Explain what happened here and how you'd interpret the results?
Transcribed Image Text:### ELISA Results Interpretation
**Question 7:** Look at the ELISA results below and answer the following questions.
**Wells Analysis:**
- **Wells 1-2:** Positive controls
- **Wells 3-7:** Samples 1-5
- **Wells 8-9:** Negative controls
**Image of ELISA Plate:**
The image shows an ELISA (Enzyme-Linked Immunosorbent Assay) plate with 9 wells filled with solutions of varying colors. The first 7 wells (from left to right) display shades of blue, while the last 2 wells are transparent.
**Color Interpretation:**
- **Positive Controls (Wells 1-2):** The blue coloration in these wells indicates a positive reaction, confirming that the assay is functioning correctly and can successfully identify the target antigen.
- **Samples (Wells 3-7):** The varying intensities of blue in these wells suggest different concentrations of the target antigen in each sample. A more intense blue color indicates a higher concentration of the antigen.
- **Negative Controls (Wells 8-9):** These wells are clear, indicating no reaction and confirming the absence of the target antigen. This ensures that there are no background interferences or false positives.
**a. Did the positive and negative controls work correctly?**
Based on the visual results:
- Yes, the positive controls (Wells 1-2) worked correctly as indicated by the blue color.
- Yes, the negative controls (Wells 8-9) also worked correctly, as evidenced by their clear (colorless) appearance, showing no reaction.
### Important Notes:
- Ensure all wells are properly labeled to avoid confusion during analysis.
- The validity of the samples' results is contingent upon appropriate positive and negative controls.
- Further quantitative analysis can be performed using a spectrophotometer to measure the absorbance of each well for precise concentration data.
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