What information do you get from these controls to give you confidence in your results? How does growth on an unopened plate affect the reliability of the other plates that were inoculated in this experiment?
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In Exercise 2-1:Ubiquity of Microorganism, we used two unopened plates as controls: one was incubated at 25° C and one was incubated at 37° C.
- What information do you get from these controls to give you confidence in your results?
- How does growth on an unopened plate affect the reliability of the other plates that were inoculated in this experiment?
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- Agarose Gel Electrophoresis Questions: 1. What determines how much agarose you should use in your gel? 2. Why do you see only DNA on the gel, and not protein? 3. How do you know which end of the gel to place the comb? 4. Suppose you turn on your power supply to run the gel and find that the milliamps reading is close to zero. What would you check?For the juice samples in exercise 3-4, you will be using the pour plate technique. In this exercise, you will add juice to a petri dish and pour agar over the sample and mix. Why is the media (potato dextrose agar, PDA) kept in a water bath until you are ready to use it, and why must you work fairly quickly? (One response answers both questions). The media is already solid and will form chunks The media has a dye added to it that must be kept at 45°C until ready to use O PDA is a media that allows growth of many types of fungi, and the heat from the water bath keeps the fungal spores from growing. The media is kept liquid in the water bath with heat, removing the tube from the heat source will cause the PDA to solidifyConsider the desired phenotype pattern of candidates that we wish to advance into experiments in Week 5 and beyond. If the growth patterns illustrated below are observed in your Week 4 results, which of the candidates would you select for further analysis? Select all that apply. Candidate A B A B C с = vigorous growth on this medium = impaired/slow growth on this medium = no growth on this medium SC-H B SC-K A B YP Gal
- Q1: Why are E.coli cells subjected to heat shock induction when the optical density of the bacterial culture has reached 0.6-0.8? Q2: Why are these wash steps critical for the preparation of protein samples used in 2D gel electrophoresis?What would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2? What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?Q24: A 7 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 31 colonies of bacteria grow on the petri dish. Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer to two decimal places using standard notation. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed. Any time more than 1 mL is transfered, a concentration is being performed.
- Experiment No. 1. Five rabbits were inoculated in the right lung and in the left side of the neck with five minims of sterilized water in which was suspended a sufficient quantity of a pure culture (third generation) of the tubercle bacillus to render the liquid quite perceptibly turbid The needle of the Koch’s inoculating syringe was inserted subcutaneously on the left side of the neck and in the third intercostal space to a depth of thirty millimetres on the right side. These animals were then confined in a small box and put in a dark cellar. They were thus deprived of light, fresh air and exercise and were also stinted in the quantity of food given them while being themselves artificially infected with the tubercle bacillus. Experiment No. 2. Five healthy rabbits were placed under the following conditions: A fresh hole about ten feet deep was dug in the middle of a field, and the animals having been confined in a small box with high sides but no top, were lowered to the bottom of…Experiment No. 1. Five rabbits were inoculated in the right lung and in the left side of the neck with five minims of sterilized water in which was suspended a sufficient quantity of a pure culture (third generation) of the tubercle bacillus to render the liquid quite perceptibly turbid The needle of the Koch’s inoculating syringe was inserted subcutaneously on the left side of the neck and in the third intercostal space to a depth of thirty millimetres on the right side. These animals were then confined in a small box and put in a dark cellar. They were thus deprived of light, fresh air and exercise and were also stinted in the quantity of food given them while being themselves artificially infected with the tubercle bacillus. Experiment No. 2. Five healthy rabbits were placed under the following conditions: A fresh hole about ten feet deep was dug in the middle of a field, and the animals having been confined in a small box with high sides but no top, were lowered to the bottom of…Refer to the provided image drawn by a student trying to plan out their serial dilution protocol. The student diluted the original culture into bottle A and then diluted it further into B as shown. The student then proceeded with plating out 0.1ml of the culture from bottle B onto the plate (Note: plate A shows the 0.1ml that was plated, no further dilutions were done). If 13 colonies grew on plate A, help the student figure out how many CFU (colony forming units) were in the original culture? Select one: a.1,300 b.13,000 c.None of the Above d.130,000 e.1,000,000
- Someone want to you an antimicrobial agent that leads 99.999% inactivation when the initial microbial load was 10^7 CFU/mL.Firstly can you determine the experimental set-up. Clarify the dilutions you made and show the viable number of CFU from each dilution.You have found that the D-value (decimal reduction value) of an antimicrobial agent to be 4 minutes when the agent was exposed to a bacterial culture having an initial total viable cel1 count of 10° CFU/mL. After 4 minutes upon addition of the antimicrobial agent, what would have been the total viable count of the culture in this experiment? A) O 10° CFU/mL B) O 10$ CFU/mL C) O 10° CFU/mL D) O 10' CFU/mLAssuming that serial dilution was carried out in a laboratory experiment in 6 tubes with 9 ml of saline in each tube and 0.1ml of the stock solution was used for initial dilution, pour plate method of inoculation was performed after the dilution process using 1ml of the culture from the samples from the test tubes. It was found out that on the 6th Petri dish after incubation, there was a total of 16 individual colonies counted. Compute the total number of microorganisms present in tube one, assuming that there was no human error in the transferring proces.