Show steps pls 1) What is the purpose in first conducting the inoculation assay prior to treatingour SY5Y cells with a chemical for a cytotoxicity assay?2) Calculate the volume of cell suspension equivalent to 200,000 cells based onthe following hemocytometer data.Cells counted: 257Squares counted: 33) You are conducing calculations for the SRB cell inoculation assay (seeprotocol). Based on the cell suspension from question 2 above, what totalvolume of cell suspension will be pipetted into each well of row A in order to setup the serial dilution scheme? Keep in mind, 1/2 this volume (and 1/2 cells!) willbe transferred down to row B as the first dilution step.4) What total volume of cells + complete medium will be pipetted from row Adown to row B? How many cells should be suspended within this transfervolume?5) Given the total volume capacity for each well of a 96-well plate is 350uL, whatvolume of complete medium will be added to row B prior to transferring cells intorow C?6) How many cells are in row H of inoculation experiment?7) Sulforhodamide B (SRB) is a cytotoxicity assay used in toxicology. In twosentences or less, explain how this assay works. 5) Given the total volume capacity for each well of a 96-well plate is 350uL, whatvolume of complete medium will be added to row B prior to transferring cells intorow C?6) How many cells are in row H of inoculation experiment?7) Sulforhodamide B (SRB) is a cytotoxicity assay used in toxicology. In twosentences or less, explain how this assay works.8) List 3 types of human error that might invalidate or compromise the inoculationassay.9) What equipment are we using to read our plates? What method is thisequipment using to give us data.10) Why do we run a blank of medium only wells?

Answered: 1) What is the purpose in first…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Show steps pls

1) What is the purpose in first conducting the inoculation assay prior to treating
our SY5Y cells with a chemical for a cytotoxicity assay?
2) Calculate the volume of cell suspension equivalent to 200,000 cells based on
the following hemocytometer data.
Cells counted: 257
Squares counted: 3
3) You are conducing calculations for the SRB cell inoculation assay (see
protocol). Based on the cell suspension from question 2 above, what total
volume of cell suspension will be pipetted into each well of row A in order to set
up the serial dilution scheme? Keep in mind, 1/2 this volume (and 1/2 cells!) will
be transferred down to row B as the first dilution step.
4) What total volume of cells + complete medium will be pipetted from row A
down to row B? How many cells should be suspended within this transfer
volume?
5) Given the total volume capacity for each well of a 96-well plate is 350uL, what
volume of complete medium will be added to row B prior to transferring cells into
row C?
6) How many cells are in row H of inoculation experiment?
7) Sulforhodamide B (SRB) is a cytotoxicity assay used in toxicology. In two
sentences or less, explain how this assay works.

5) Given the total volume capacity for each well of a 96-well plate is 350uL, what
volume of complete medium will be added to row B prior to transferring cells into
row C?
6) How many cells are in row H of inoculation experiment?
7) Sulforhodamide B (SRB) is a cytotoxicity assay used in toxicology. In two
sentences or less, explain how this assay works.
8) List 3 types of human error that might invalidate or compromise the inoculation
assay.
9) What equipment are we using to read our plates? What method is this
equipment using to give us data.
10) Why do we run a blank of medium only wells?

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Disclaimer: "Bartleby guidelines allow the first question to be solved. And please ask other questions separately."

Introduction: Cytotoxicity is a test that is used to measure the toxic effects of various substances on living cells. It is used in toxicology and pharmacology to understand how drugs and chemicals affect cell viability and function. There are various methods for conducting cytotoxicity assays, which are as follows:

The trypan blue exclusion method involves staining non-viable cells with trypan blue, which can be visually counted under a microscope.
The Lamar blue assay measures cell metabolic activity by monitoring the reduction of a redox indicator dye.
CCK kits use colorimetric reagents to assess cell viability based on metabolic activity.
The LDH release assay quantifies the release of lactate dehydrogenase from damaged cells, which indicates cellular damage.

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