The first choice : ori lacZ blaz araC GFP The second choice : arabinose Ampicillin X-gal IPTG GFP The third choice : X-gal lactose arabinose GFP Ampicillin The fourth choice : on ice at 42℃ at 95℃ at 37℃ at RT The fifth choice : arabinose ampicillin lactose X-gal GFP
The first choice : ori lacZ blaz araC GFP The second choice : arabinose Ampicillin X-gal IPTG GFP The third choice : X-gal lactose arabinose GFP Ampicillin The fourth choice : on ice at 42℃ at 95℃ at 37℃ at RT The fifth choice : arabinose ampicillin lactose X-gal GFP
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
The first choice :
ori
lacZ
blaz
araC
GFP
The second choice :
arabinose
Ampicillin
X-gal
IPTG
GFP
The third choice :
X-gal
lactose
arabinose
GFP
Ampicillin
The fourth choice :
on ice
at 42℃
at 95℃
at 37℃
at RT
The fifth choice :
arabinose
ampicillin
lactose
X-gal
GFP

Transcribed Image Text:41
The image shown is the result from an experiment involving a plasmid carrying the Select alternative ✓ gene
which also contains a site that can be cleaved by restriction enzymes to allow a DNA fragments of interest to be
inserted. In the presence of Select alternative ✓ , the beta-galactosidase enzyme acts on the substrate
Select alternative to produce a coloured product.
After ligation of a DNA fragment of interest into the plasmid the ligation mix was introduced into E. coli cells using
temperature treatment Select alternative. The transformed cells are later spread onto agar plates in the
presence of Select alternative to select for the plasmid, and the components that produce the colour reaction.
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