The first choice : ori lacZ blaz araC GFP The second choice : arabinose Ampicillin  X-gal IPTG GFP The third choice : X-gal lactose arabinose GFP Ampicillin The fourth choice : on ice at 42℃ at 95℃ at 37℃ at RT The fifth choice : arabinose ampicillin lactose X-gal GFP

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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The first choice :

ori

lacZ

blaz

araC

GFP

The second choice :

arabinose

Ampicillin

 X-gal

IPTG

GFP

The third choice :

X-gal

lactose

arabinose

GFP

Ampicillin

The fourth choice :

on ice

at 42℃

at 95℃

at 37℃

at RT

The fifth choice :

arabinose

ampicillin

lactose

X-gal

GFP

41
The image shown is the result from an experiment involving a plasmid carrying the Select alternative ✓ gene
which also contains a site that can be cleaved by restriction enzymes to allow a DNA fragments of interest to be
inserted. In the presence of Select alternative ✓ , the beta-galactosidase enzyme acts on the substrate
Select alternative to produce a coloured product.
After ligation of a DNA fragment of interest into the plasmid the ligation mix was introduced into E. coli cells using
temperature treatment Select alternative. The transformed cells are later spread onto agar plates in the
presence of Select alternative to select for the plasmid, and the components that produce the colour reaction.
Transcribed Image Text:41 The image shown is the result from an experiment involving a plasmid carrying the Select alternative ✓ gene which also contains a site that can be cleaved by restriction enzymes to allow a DNA fragments of interest to be inserted. In the presence of Select alternative ✓ , the beta-galactosidase enzyme acts on the substrate Select alternative to produce a coloured product. After ligation of a DNA fragment of interest into the plasmid the ligation mix was introduced into E. coli cells using temperature treatment Select alternative. The transformed cells are later spread onto agar plates in the presence of Select alternative to select for the plasmid, and the components that produce the colour reaction.
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