Identify the method of inoculation to the test. Phenylalanine Deaminase Test SIM Medium Stab agar with inoculating loop many times Stab agar with inoculating loop many times Inoculate slant with wire loop Urea hydrolysis test (urease) Gelatin hydrolysis test (gelatinase)
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- Special Media for Isolating Bacteria OBJECTIVES Because multiple methods and multiple media exist, you must be able to match the correct procedure to the desired microbe. For example, if bacterium B is salt-tolerant, a high concentration (>5%) of salt could be added to the culture medium. Physical conditions can also be used to select for a bacterium. If bacteri- After completing this exercise, you should be able to: 1. Differentiate selective from differential media. 2. Provide an application for enrichment and selec- tive media. um B is heat-resistant, the specimen could be heated before isolation. Dyes such as phenol red, eosin, or methylene blue are sometimes ineluded in differential BACKGROUND media. Products of bacterial metabolism can react with One of the major limitations of dilution techniques used to isolate bacteria is that organisms present in limited amounts may be diluted out on plates filled with dominant bacteria. For example, if the culture to be isolated has 1…Can you please help me describe the colony apperance on these two stains? Thank you! Growth on Blood Agar - 48 hours Growth on Chocolate Agar Results: Growth on Blood Agar 48 hours Growth on Chocolate AgarWhat is the color result of 1% solution of casein, Albumin, Gelatin in ninhydrin test? Include picture and interpretation With reference
- Create a flow chart to perform the required tests to identify unknown microorganism: - Use at least 3 other biochemical tests from the following list: Mannitol salt agar, Blood agar, Starch agar, Tributyrin Agar, Gelatin, Casein agar, Indole Production, MR-VP, Citrate, Hydrogen Sulfide test, Urease test, Nitrate reduction test, Catalase test, and Oxidase test. The Gram positive organisms that could be present in your unknown mixture are: Bacillus cereus, Micrococcus luteus, Staphylococcus aureus, Staphylococcus epidermidis and Lactococcus lactis.Why is the looping out method preferable for sputum specimen for acid fast smears?The figure attached shows the colonies on Eosin Methylene Blue Agar (EMBA) plate. Make a description on the picture.
- A bacteria will appear pinkish to orange if it is positive for UREASE test True FalseSalmonella-Shigella (SS) agar plate results (kitchen sink drain and kitchen dish cloth) Using the following information about SS agar and the image below, describe and identify the growth on the SS agar plate in the table on page 6. SS agar plate - results of kitchen sink drain and kitchen dish cloth (plate below) Salmonella-Shigella (SS) agar: Salmonella-Shigella agar (SS agar) is a selective and differential medium utilized for the selection and characterization pathogenic enteric bacteria. SS agar contains lactose, bile salts, ferric citrate, and neutral red. Kitchen The bile salts select for Gram-negative bacteria, while inhibiting Gram-positive bacteria. sink drain The other components differentiate among Gram-negative bacteria. Coliform bacteria such as E. coli will ferment the lactose in the media, resulting in bacterial growth with a pink color. (They do not produce any hydrogen sulfide.) Members of the genus Salmonella will not ferment lactose, but do product hydrogen sulfide…What is the unknownn organism based on this biochemical test results? Gram Stain: Gram Positive Oxidase: Positive Catalase: Positive MAC: Non Lactose Fermenter MSA: Mannitol Salt Fermenter Gelatin Hydrolysis: Negative Indole Production: Negative Motility Indole Ornithine Medium: Negative MR/VP Test: Negative Nitrate Test: Positive Simmon Citrate Test: Negative Urease Test: Positive Possible Organisms: Vibrio fischeri Enterococcus faecalis Micrococcus luteus Streptococcus pneumoniae Pseudomonas aeruginosa Proteus mirabilis Acinetobacter baumannii Staphylococcus aureus Mycoplasma pneumoniae Escherichia coli Candida albicans Salmonella enterica Listeria monocytogenes