A gel filtration column was calibrated by measuring elution volumes for two proteins of known mass: glutamate dehydrogenase, 290 kDa with elution volume 20.8 mL; serum albumin, 37 kDa with elution volume 42.5 mL Use these values to determine the equation of the straight line graph that relates log molar mass to elution volume. The elution volume of an unknown protein was 32.2 mL. Use the equation just determined above to estimate the molar mass of the protein in kDa units. Don't round your answer until the end to ensure your answer is within the accepted range. Round your answer to the nearest whole number (e.g. If you calculated 27.52, input your answer as 28).
A gel filtration column was calibrated by measuring elution volumes for two proteins of known mass: glutamate dehydrogenase, 290 kDa with elution volume 20.8 mL; serum albumin, 37 kDa with elution volume 42.5 mL
Use these values to determine the equation of the straight line graph that relates log molar mass to elution volume.
The elution volume of an unknown protein was 32.2 mL. Use the equation just determined above to estimate the molar mass of the protein in kDa units.
Don't round your answer until the end to ensure your answer is within the accepted range.
Round your answer to the nearest whole number (e.g. If you calculated 27.52, input your answer as 28).
The established amounts of the elution volumes for the two proteins with determined masses can be used to calculate the formula of a straight-line graph that links log molar mass and elution volume. Then, depending on the protein's elution volume, one can employ this equation to calculate the protein's molar mass.
Step by step
Solved in 3 steps with 6 images
