can someone explain these calculations to me?
At first, a standard curve is made using the OD of protein of known concentrations, i.e., ODs mentioned for blank to 20ug/ml protein concentration and a linear trend line is obtained with the equation mentioned in step 2. This equation is compared with the straight line equation "Y=mX+c" where Y is the OD, X is protein concentration, m is the tangent/slope of the line and c is the intercept. We can calculate unknown X (concentration) using this equation if we have the Y(OD) value by modifying the formula to X="(Y-c)/m".
Now we have a protein solution of unknown concentration that has been diluted 50 times and 10ul of the diluted sample has been used to obtain OD. Using the trendline equation mentioned earlier, the protein content of the 10ul diluted sample is calculated to be 16.57.
Further, the protein content for 1 ml solution is calculated by multiplying with 100 and the concentration of diluted sample expressed in ug/ml.
Since the original sample was diluted 50 times in the first place, the concentration is multiplied again by 50 and the original concentration of the protein sample is obtained.
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