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You have a 20% stock of NaCl, a 30% stack of KHPO, 2.5 M NH CI and 10 mg/ml stock of ampicill a. How many grams of NaCl would you need to weigh out to make up 250 ml of a solution? 20

You have a 20% stock of NaCl, a 30% stack of KHPO, 2.5 M NH CI and 10 mg/ml stock of ampicill a. How many grams of NaCl would you need to weigh out to make up 250 ml of a solution? 20

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question 6a and 6b explained 

6 You have a 20% srock of NaCl, a 30% stack of KHPO, 25 M NH,Cl and 10 mg/ml stock of ampicillin. a. How many grams of NaCl would you need to weigh out to make up 250 ml of a solution? 20 I000 b. You will be preparing 300 ml of media. You need to dilute this 20% stock of NaCl, the 30% stock of K HPO, the 2.5 M NH CI and the 10 mg/ml ampicillin to prepare your media. You will need 1.5% NaCl and 2% K,HPO,, 0.1 M NH,Cl and 0.05 mg/ml ampicillin. Calculate how much stocks you need to add. Fill in the table below. Ct Stock Final Concentration needed ml added 1.5x300/200x.100= 1.5% •225 20% NaCI 2x300/30x100 30% К НРО, 2% .1x 3 D0/2.5 K.1Do. .012 2.5 M NH,CI 0.1M .o15 10 mg/ml ampicillin 0.05 mg/ml 299.548 To 300 ml water
Transcribed Image Text:6 You have a 20% srock of NaCl, a 30% stack of KHPO, 25 M NH,Cl and 10 mg/ml stock of ampicillin. a. How many grams of NaCl would you need to weigh out to make up 250 ml of a solution? 20 I000 b. You will be preparing 300 ml of media. You need to dilute this 20% stock of NaCl, the 30% stock of K HPO, the 2.5 M NH CI and the 10 mg/ml ampicillin to prepare your media. You will need 1.5% NaCl and 2% K,HPO,, 0.1 M NH,Cl and 0.05 mg/ml ampicillin. Calculate how much stocks you need to add. Fill in the table below. Ct Stock Final Concentration needed ml added 1.5x300/200x.100= 1.5% •225 20% NaCI 2x300/30x100 30% К НРО, 2% .1x 3 D0/2.5 K.1Do. .012 2.5 M NH,CI 0.1M .o15 10 mg/ml ampicillin 0.05 mg/ml 299.548 To 300 ml water
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