Question 6a and 6b explained 6 You have a 20% srock of NaCl, a 30% stack of KHPO, 25 M NH,Cl and 10 mg/ml stock of ampicillin.a. How many grams of NaCl would you need to weigh out to make up 250 ml of a solution?20I000b. You will be preparing 300 ml of media. You need to dilute this 20% stock of NaCl, the30% stock of K HPO, the 2.5 M NH CI and the 10 mg/ml ampicillin to prepare your media.You will need 1.5% NaCl and 2% K,HPO,, 0.1 M NH,Cl and 0.05 mg/ml ampicillin. Calculatehow much stocks you need to add. Fill in the table below.CtStockFinal Concentration neededml added1.5x300/200x.100=1.5%•22520% NaCI2x300/30x10030% К НРО,2%.1x 3 D0/2.5 K.1Do..0122.5 M NH,CI0.1M.o1510 mg/ml ampicillin0.05 mg/ml299.548To 300 mlwater

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Answered: You have a 20% stock of NaCl, a 30%…

You have a 20% stock of NaCl, a 30% stack of KHPO, 2.5 M NH CI and 10 mg/ml stock of ampicill a. How many grams of NaCl would you need to weigh out to make up 250 ml of a solution? 20

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

Question 6a and 6b explained

6 You have a 20% srock of NaCl, a 30% stack of KHPO, 25 M NH,Cl and 10 mg/ml stock of ampicillin.
a. How many grams of NaCl would you need to weigh out to make up 250 ml of a solution?
20
I000
b. You will be preparing 300 ml of media. You need to dilute this 20% stock of NaCl, the
30% stock of K HPO, the 2.5 M NH CI and the 10 mg/ml ampicillin to prepare your media.
You will need 1.5% NaCl and 2% K,HPO,, 0.1 M NH,Cl and 0.05 mg/ml ampicillin. Calculate
how much stocks you need to add. Fill in the table below.
Ct
Stock
Final Concentration needed
ml added
1.5x300/200x.100=
1.5%
•225
20% NaCI
2x300/30x100
30% К НРО,
2%
.1x 3 D0/2.5 K.1Do.
.012
2.5 M NH,CI
0.1M
.o15
10 mg/ml ampicillin
0.05 mg/ml
299.548
To 300 ml
water

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