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can you show how to calculate the amount of two components for the required volume of a buffer with the receipes? Prepare enough 10X stock solution  § Prepare 1L Proportions § 0.25M tris § 1.92M glycine § pH 8.3 § 1% (w/v) SDS Instructions to prepare 100mL of 10X running buffer 1. Weigh 3.02g of Tris base and 14.42g of glycine into ~90mL of ddH2O in a clean beaker and stir to dissolve. 2. Check the pH with a calibrated pH metre once dissolved. § The pH of the buffer should be 8.3. pH adjustment is not required if between 8.1-8.5. 3. Add 5mL 20% (w/v) SDS stock solution (a liquid) to make a 1% (w/v) solution. 4. Top the volume to 100mL by adding 5mL ddH2O from a graduated cylinder.

can you show how to calculate the amount of two components for the required volume of a buffer with the receipes? Prepare enough 10X stock solution  § Prepare 1L Proportions § 0.25M tris § 1.92M glycine § pH 8.3 § 1% (w/v) SDS Instructions to prepare 100mL of 10X running buffer 1. Weigh 3.02g of Tris base and 14.42g of glycine into ~90mL of ddH2O in a clean beaker and stir to dissolve. 2. Check the pH with a calibrated pH metre once dissolved. § The pH of the buffer should be 8.3. pH adjustment is not required if between 8.1-8.5. 3. Add 5mL 20% (w/v) SDS stock solution (a liquid) to make a 1% (w/v) solution. 4. Top the volume to 100mL by adding 5mL ddH2O from a graduated cylinder.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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can you show how to calculate the amount of two components for the required volume of a buffer with the receipes?

Prepare enough 10X stock solution 


§ Prepare 1L
Proportions
§ 0.25M tris
§ 1.92M glycine
§ pH 8.3
§ 1% (w/v) SDS
Instructions to prepare 100mL of 10X running buffer
1. Weigh 3.02g of Tris base and 14.42g of glycine into ~90mL of ddH2O in a clean beaker and stir to
dissolve.
2. Check the pH with a calibrated pH metre once dissolved.
§ The pH of the buffer should be 8.3. pH adjustment is not required if between 8.1-8.5.
3. Add 5mL 20% (w/v) SDS stock solution (a liquid) to make a 1% (w/v) solution.
4. Top the volume to 100mL by adding 5mL ddH2O from a graduated cylinder.

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