Protein concentration standards 0.6 0.5 0.4 0.3 0.2 0.1 0.5 1 1.5 2.5 Albumin concentration (mg/mL) A standard curve of known concentrations of protein were reacted with the Biuret reagent. A linear curve fit is presented above. Use the graph to estimate the concentration of an unknown sample with an Ass0 value of 0.416 (A rough estimate is fine. You do not need a calculator for this problem) • Use the graph to estimate the concentration of an unknown sample with an A550 value of 0.233 • Which instrument did we use to measure the intensity of the color of proteins that reacted with Biuret reagent? OSSV

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