1/ Final [PNPP] (mM-1) 2.5 2 1.5 0.5 0 Km (mm) Vmax (μM/min) Lineweaver-Burk (LB) plot 1/ Final [PNPP] (mM-1) vs 1/VO (min/µM) y = 7.9966x -0.6983 Buffer 1 0.2 0.4 0.6 0.8 1/V0 (min/μM) y = 1.6343x -0.0839 Buffer 2 1 6. Determine the Km and Vmax from the LB plot for enzyme assays in buffer 1 and buffer 2. Buffer 1 Buffer 2 1.2 1.4

1/ Final [PNPP] (mM-1) 2.5 2 1.5 0.5 0 Km (mm) Vmax (μM/min) Lineweaver-Burk (LB) plot 1/ Final [PNPP] (mM-1) vs 1/VO (min/µM) y = 7.9966x -0.6983 Buffer 1 0.2 0.4 0.6 0.8 1/V0 (min/μM) y = 1.6343x -0.0839 Buffer 2 1 6. Determine the Km and Vmax from the LB plot for enzyme assays in buffer 1 and buffer 2. Buffer 1 Buffer 2 1.2 1.4

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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