You wish to determine the %concentration of a stock solution of brilliant blue FCF(MW=792.8grams/mole; extinction coefficient,25=97000M1cm 1).You take 5ul of the stock and dilute up to 1ml. You take 10ul of this dilution an dilute it up to 500ul.Finally you take 1ul from this dilution and dilute it up to 1ml in a 1cm diameter cuvette.You obtain an As91=0.001 SHOW YOUR WORK. Do not forget units.1. What is your total dilution factor?2. What is the molar concentration of the dye in the original stock?3. What is the % concentration of the dye in the original stock?

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Description: You wish to determine the %concentration of a stock solution of brilliant blue FCF(MW=792.8grams/mole; extinction coefficient,25=97000M1cm 1).You take 5ul of the stock and dilute up to 1ml. You take 10ul of this dilution an dilute it up to 500ul.Fin

Absorption spectroscopy is a spectroscopic technique in which we measure the absorption of radiation…

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1. What is your total dilution factor? 2. What is the molar concentration of the dye in the original stock? 3. What is the % concentration of the dye in the original stock?

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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You wish to determine the %concentration of a stock solution of brilliant blue FCF
(MW=792.8grams/mole; extinction coefficient,25=97000M1cm 1).
You take 5ul of the stock and dilute up to 1ml. You take 10ul of this dilution an dilute it up to 500ul.
Finally you take 1ul from this dilution and dilute it up to 1ml in a 1cm diameter cuvette.
You obtain an As91=0.001 SHOW YOUR WORK. Do not forget units.
1. What is your total dilution factor?
2. What is the molar concentration of the dye in the original stock?
3. What is the % concentration of the dye in the original stock?

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