A student performed an invertase activity assay on samples from a purification.  All reactions occurred in a reaction volume of 100 µL and lasted 3.1 minutes.  For each reaction, 5.00 µL enzyme was mixed with 50.0 mM sucrose in 10.0 mM acetate pH 4.8.  Then 400 µL of DNS was added, the samples were heated to 90 °C for 5 minutes, and 500 µL of 50.0 mM acetate pH 4.8 was added before loading a 96 well plate in triplicate.  Samples were measured at 540 nm using a path length of 0.680 cm.  The fraction with the highest activity had A540 of 0.904.  The standard of 14.00 mM hydrolyzed sucrose had A540 of 1.047 (the concentration given is of the 100 µL sample).  The no-enzyme control had A540 of 0.174.  Calculate the activity of the fraction (in nmol min-1).

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Scientific Notation

We have to deal with quantities that are extremely large or very small. For example- I may have 6,500,000,000 red blood cells, or the diameter of an iron atom is 0.000000011 inches. Numbers with many zeros can be inexpedient to work with, so scientists use scientific notation. It represents very large numbers or very small numbers in a simple form. We know that whole numbers can be extended to infinity, but we cannot write such large numbers on paper. Numbers that present in millions of places after the decimal point need to be represented simply. Thus, it is difficult to represent a few numbers in their extended form. Therefore, we use scientific notation.