Both laboratories used 10 micrograms of protein each in their kinetic assays. Protein concentrations were determined by the Bradford protein assay. Assay conditions employed in the two labs (pH, temperature, etc.) were also identical. What would be the most plausible cause for the discrepancy in the Vmax values for the compound I? Explain. Recall that the Bradford assay measures total protein amounts in sample solution based on complex formation between a dye and proteins. Also, the assay solution used in both labs does not contain any inhibitors.
Proteins
We generally tend to think of proteins only from a dietary lens, as a component of what we eat. However, they are among the most important and abundant organic macromolecules in the human body, with diverse structures and functions. Every cell contains thousands and thousands of proteins, each with specific functions. Some help in the formation of cellular membrane or walls, some help the cell to move, others act as messages or signals and flow seamlessly from one cell to another, carrying information.
Protein Expression
The method by which living organisms synthesize proteins and further modify and regulate them is called protein expression. Protein expression plays a significant role in several types of research and is highly utilized in molecular biology, biochemistry, and protein research laboratories.
(b) Both laboratories used 10 micrograms of protein each in their kinetic assays. Protein concentrations were
determined by the Bradford protein assay. Assay conditions employed in the two labs (pH, temperature,
etc.) were also identical. What would be the most plausible cause for the discrepancy in the Vmax values
for the compound I? Explain.
Recall that the Bradford assay measures total protein amounts in sample solution based on complex
formation between a dye and proteins. Also, the assay solution used in both labs does not contain any
inhibitors.
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