Droplet based single cell DNA sequencing uses a microfluidic platform which generates droplets encapsulating individual cells. A micrograph of such a device [Zilionis et al, Nature Protocols 2016] is presented in Figure Q3. The channels are 25 µm deep. (a) (b) - (c) Oil Figure Q3. Micrograph of the microfluidic droplet device. Scale bars are 100 µm. Adapted from Zilionis et al, Nature Protocols 2016. Red arrowheads show individual cells, and black arrows indicate flow direction. RT/lysis reagents Cells Barcoding hydrogel beads A company wants to explore the design of a prototype of the device made from PDMS. Describe a suitable fabrication process, explaining the rationale behind each individual step. Schematics can be drawn to illustrate your response. You may want to refer to the datasheets at the end of the script. Testing of the prototype was successful and the company wants to explore the feasibility of manufacturing the device from glass. Describe the fabrication process for this material, explaining the rationale behind each individual step. Schematics can be drawn to illustrate your response. You may want to refer to the datasheets at the end of the script. The new product is a success, so the company decides to further develop the device and manufacture it in a polymer using injection moulding. Describe the fabrication process in this case along with the rationale behind each step. Schematics can be drawn to illustrate your response. You may want to refer to the datasheets at the end of the script.
Droplet based single cell DNA sequencing uses a microfluidic platform which generates droplets encapsulating individual cells. A micrograph of such a device [Zilionis et al, Nature Protocols 2016] is presented in Figure Q3. The channels are 25 µm deep. (a) (b) - (c) Oil Figure Q3. Micrograph of the microfluidic droplet device. Scale bars are 100 µm. Adapted from Zilionis et al, Nature Protocols 2016. Red arrowheads show individual cells, and black arrows indicate flow direction. RT/lysis reagents Cells Barcoding hydrogel beads A company wants to explore the design of a prototype of the device made from PDMS. Describe a suitable fabrication process, explaining the rationale behind each individual step. Schematics can be drawn to illustrate your response. You may want to refer to the datasheets at the end of the script. Testing of the prototype was successful and the company wants to explore the feasibility of manufacturing the device from glass. Describe the fabrication process for this material, explaining the rationale behind each individual step. Schematics can be drawn to illustrate your response. You may want to refer to the datasheets at the end of the script. The new product is a success, so the company decides to further develop the device and manufacture it in a polymer using injection moulding. Describe the fabrication process in this case along with the rationale behind each step. Schematics can be drawn to illustrate your response. You may want to refer to the datasheets at the end of the script.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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DNA and RNA
Deoxyribonucleic acid (DNA) is usually called the blueprint of life. Deoxyribose is a monosaccharide that has a key function in the synthesis of deoxyribonucleic acid. One less oxygen-containing hydroxyl group occurs in deoxyribose sugar. Nucleic acid, deoxyribonucleic acid, is one of the natural components. Deoxyribonucleic acid is a double-stranded molecule. Watson and Crick postulated the double-stranded model of the helix. A deoxyribonucleic acid is a molecular group that carries and transmits genetic information from parents to offspring. All eukaryotic and prokaryotic cells are involved.
DNA as the Genetic Material
DNA, or deoxyribonucleic acid, is a long polymeric nucleic acid molecule discovered in the late 1930s. It is a polymer; a long chain-like molecule made up of several monomers connected in a sequence. It possesses certain characteristics that qualify it as a genetic component. Certain organisms have different types of nucleic acids as their genetic material - DNA or RNA.
Genetics
The significant branch in science which involves the study of genes, gene variations, and the organism's heredity is known as genetics. It is also used to study the involvement of a gene or set of genes in the health of an individual and how it prevents several diseases in a human being. Thus, genetics also creates an understanding of various medical conditions.
DNA Replication
The mechanism by which deoxyribonucleic acid (DNA) is capable of producing an exact copy of its own is defined as DNA replication. The DNA molecules utilize a semiconservative method for replication.
Question
![Droplet based single cell DNA sequencing uses a microfluidic platform which
generates droplets encapsulating individual cells. A micrograph of such a device
[Zilionis et al, Nature Protocols 2016] is presented in Figure Q3. The channels are
25 μm deep.
(a)
(b)
Oil
Figure Q3. Micrograph of the microfluidic droplet device. Scale bars are 100 µm.
Adapted from Zilionis et al, Nature Protocols 2016. Red arrowheads show
individual cells, and black arrows indicate flow direction.
(c)
RT/lysis
reagents
Cells
Barcoding hydrogel beads
A company wants to explore the design of a prototype of the device made from
PDMS. Describe a suitable fabrication process, explaining the rationale behind
each individual step. Schematics can be drawn to illustrate your response. You
may want to refer to the datasheets at the end of the script.
Testing of the prototype was successful and the company wants to explore the
feasibility of manufacturing the device from glass. Describe the fabrication
process for this material, explaining the rationale behind each individual step.
Schematics can be drawn to illustrate your response. You may want to refer to
the datasheets at the end of the script.
The new product is a success, so the company decides to further develop the
device and manufacture it in a polymer using injection moulding. Describe the
fabrication process in this case along with the rationale behind each step.
Schematics can be drawn to illustrate your response. You may want to refer to
the datasheets at the end of the script.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F0d77d484-a887-469d-aaae-779d8340e1ea%2Fcfb39c37-1447-4c35-bd2f-892d3c57c8d3%2Fb4zui1j_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Droplet based single cell DNA sequencing uses a microfluidic platform which
generates droplets encapsulating individual cells. A micrograph of such a device
[Zilionis et al, Nature Protocols 2016] is presented in Figure Q3. The channels are
25 μm deep.
(a)
(b)
Oil
Figure Q3. Micrograph of the microfluidic droplet device. Scale bars are 100 µm.
Adapted from Zilionis et al, Nature Protocols 2016. Red arrowheads show
individual cells, and black arrows indicate flow direction.
(c)
RT/lysis
reagents
Cells
Barcoding hydrogel beads
A company wants to explore the design of a prototype of the device made from
PDMS. Describe a suitable fabrication process, explaining the rationale behind
each individual step. Schematics can be drawn to illustrate your response. You
may want to refer to the datasheets at the end of the script.
Testing of the prototype was successful and the company wants to explore the
feasibility of manufacturing the device from glass. Describe the fabrication
process for this material, explaining the rationale behind each individual step.
Schematics can be drawn to illustrate your response. You may want to refer to
the datasheets at the end of the script.
The new product is a success, so the company decides to further develop the
device and manufacture it in a polymer using injection moulding. Describe the
fabrication process in this case along with the rationale behind each step.
Schematics can be drawn to illustrate your response. You may want to refer to
the datasheets at the end of the script.
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