SHEET 7-1 SEQUENCE 10b 11 12 PROCEDURAL STEP NOTE: Sample color provides a good indication of what wavelength region to use. A yellow solution absorbs light in the 400- to 500-nm region. A red solution absorbs light between 500 and 600 nm, and blue solutions absorb light in the 600- to 700-nm range. A nanometer (nm) equals 10-8 meter. When actually doing an assay with high and low concentration, check to be sure there is enough difference in absorbance between low and high analyte concentrations by measuring other standard solutions with expected low and high concentrations. Remove the final cuvette from the spectrophotometer, carefully rinse the cuvettes with distilled water. Store spectrophotometer cuvettes, keeping them separate from regular test tubes. If the instrument is stored on the benchtop, cover with a dust cover. Otherwise, unplug and place on a storage shelf. INSTRUCTOR- OBSERVED ACCEPTABLE PERFORMANCE (CHECK IF ACCEPTABLE) Procedural Evaluation Student's Name Instructor's Signature Comments: Grade Date TABLE 1A WAVELENGTH (nm) ABSORBANCE TABLE 1B WAVELENGTH ABSORBANCE 550 0.477 512 0.769 500 0.762 511 0.773 450 0.355 400 0.134 510 0.780 509 0.787 WAVELENGTH (nm) ABSORBANCE 508 0.781 520 0.748 507 0.764 515 0.759 510 0.780 505 0.771 500 0.771 495 0.651 490 0.590
SHEET 7-1 SEQUENCE 10b 11 12 PROCEDURAL STEP NOTE: Sample color provides a good indication of what wavelength region to use. A yellow solution absorbs light in the 400- to 500-nm region. A red solution absorbs light between 500 and 600 nm, and blue solutions absorb light in the 600- to 700-nm range. A nanometer (nm) equals 10-8 meter. When actually doing an assay with high and low concentration, check to be sure there is enough difference in absorbance between low and high analyte concentrations by measuring other standard solutions with expected low and high concentrations. Remove the final cuvette from the spectrophotometer, carefully rinse the cuvettes with distilled water. Store spectrophotometer cuvettes, keeping them separate from regular test tubes. If the instrument is stored on the benchtop, cover with a dust cover. Otherwise, unplug and place on a storage shelf. INSTRUCTOR- OBSERVED ACCEPTABLE PERFORMANCE (CHECK IF ACCEPTABLE) Procedural Evaluation Student's Name Instructor's Signature Comments: Grade Date TABLE 1A WAVELENGTH (nm) ABSORBANCE TABLE 1B WAVELENGTH ABSORBANCE 550 0.477 512 0.769 500 0.762 511 0.773 450 0.355 400 0.134 510 0.780 509 0.787 WAVELENGTH (nm) ABSORBANCE 508 0.781 520 0.748 507 0.764 515 0.759 510 0.780 505 0.771 500 0.771 495 0.651 490 0.590
Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter3: Biosignals And Noise
Section: Chapter Questions
Problem 10Q
Related questions
Question
None
Transcribed Image Text:SHEET 7-1
SEQUENCE
10b
11
12
PROCEDURAL STEP
NOTE: Sample color provides a good indication of what wavelength region to use.
A yellow solution absorbs light in the 400- to 500-nm region. A red solution absorbs
light between 500 and 600 nm, and blue solutions absorb light in the 600- to 700-nm
range.
A nanometer (nm) equals 10-8 meter.
When actually doing an assay with high and low concentration, check to be sure there
is enough difference in absorbance between low and high analyte concentrations by
measuring other standard solutions with expected low and high concentrations.
Remove the final cuvette from the spectrophotometer, carefully rinse the cuvettes
with distilled water.
Store spectrophotometer cuvettes, keeping them separate from regular test tubes.
If the instrument is stored on the benchtop, cover with a dust cover. Otherwise,
unplug and place on a storage shelf.
INSTRUCTOR-
OBSERVED
ACCEPTABLE
PERFORMANCE
(CHECK IF
ACCEPTABLE)
Procedural Evaluation
Student's Name
Instructor's Signature
Comments:
Grade
Date
TABLE 1A
WAVELENGTH (nm)
ABSORBANCE
TABLE 1B
WAVELENGTH
ABSORBANCE
550
0.477
512
0.769
500
0.762
511
0.773
450
0.355
400
0.134
510
0.780
509
0.787
WAVELENGTH (nm)
ABSORBANCE
508
0.781
520
0.748
507
0.764
515
0.759
510
0.780
505
0.771
500
0.771
495
0.651
490
0.590
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 2 steps
Recommended textbooks for you
Principles Of Radiographic Imaging: An Art And A …
Health & Nutrition
ISBN:
9781337711067
Author:
Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:
Cengage Learning
Principles Of Radiographic Imaging: An Art And A …
Health & Nutrition
ISBN:
9781337711067
Author:
Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:
Cengage Learning
Case Studies In Health Information Management
Biology
ISBN:
9781337676908
Author:
SCHNERING
Publisher:
Cengage