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SHEET 7-1 SEQUENCE 10b 11 12 PROCEDURAL STEP NOTE: Sample color provides a good indication of what wavelength region to use. A yellow solution absorbs light in the 400- to 500-nm region. A red solution absorbs light between 500 and 600 nm, and blue solutions absorb light in the 600- to 700-nm range. A nanometer (nm) equals 10-8 meter. When actually doing an assay with high and low concentration, check to be sure there is enough difference in absorbance between low and high analyte concentrations by measuring other standard solutions with expected low and high concentrations. Remove the final cuvette from the spectrophotometer, carefully rinse the cuvettes with distilled water. Store spectrophotometer cuvettes, keeping them separate from regular test tubes. If the instrument is stored on the benchtop, cover with a dust cover. Otherwise, unplug and place on a storage shelf. INSTRUCTOR- OBSERVED ACCEPTABLE PERFORMANCE (CHECK IF ACCEPTABLE) Procedural Evaluation Student's Name Instructor's Signature Comments: Grade Date TABLE 1A WAVELENGTH (nm) ABSORBANCE TABLE 1B WAVELENGTH ABSORBANCE 550 0.477 512 0.769 500 0.762 511 0.773 450 0.355 400 0.134 510 0.780 509 0.787 WAVELENGTH (nm) ABSORBANCE 508 0.781 520 0.748 507 0.764 515 0.759 510 0.780 505 0.771 500 0.771 495 0.651 490 0.590

SHEET 7-1 SEQUENCE 10b 11 12 PROCEDURAL STEP NOTE: Sample color provides a good indication of what wavelength region to use. A yellow solution absorbs light in the 400- to 500-nm region. A red solution absorbs light between 500 and 600 nm, and blue solutions absorb light in the 600- to 700-nm range. A nanometer (nm) equals 10-8 meter. When actually doing an assay with high and low concentration, check to be sure there is enough difference in absorbance between low and high analyte concentrations by measuring other standard solutions with expected low and high concentrations. Remove the final cuvette from the spectrophotometer, carefully rinse the cuvettes with distilled water. Store spectrophotometer cuvettes, keeping them separate from regular test tubes. If the instrument is stored on the benchtop, cover with a dust cover. Otherwise, unplug and place on a storage shelf. INSTRUCTOR- OBSERVED ACCEPTABLE PERFORMANCE (CHECK IF ACCEPTABLE) Procedural Evaluation Student's Name Instructor's Signature Comments: Grade Date TABLE 1A WAVELENGTH (nm) ABSORBANCE TABLE 1B WAVELENGTH ABSORBANCE 550 0.477 512 0.769 500 0.762 511 0.773 450 0.355 400 0.134 510 0.780 509 0.787 WAVELENGTH (nm) ABSORBANCE 508 0.781 520 0.748 507 0.764 515 0.759 510 0.780 505 0.771 500 0.771 495 0.651 490 0.590

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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SHEET 7-1 SEQUENCE 10b 11 12 PROCEDURAL STEP NOTE: Sample color provides a good indication of what wavelength region to use. A yellow solution absorbs light in the 400- to 500-nm region. A red solution absorbs light between 500 and 600 nm, and blue solutions absorb light in the 600- to 700-nm range. A nanometer (nm) equals 10-8 meter. When actually doing an assay with high and low concentration, check to be sure there is enough difference in absorbance between low and high analyte concentrations by measuring other standard solutions with expected low and high concentrations. Remove the final cuvette from the spectrophotometer, carefully rinse the cuvettes with distilled water. Store spectrophotometer cuvettes, keeping them separate from regular test tubes. If the instrument is stored on the benchtop, cover with a dust cover. Otherwise, unplug and place on a storage shelf. INSTRUCTOR- OBSERVED ACCEPTABLE PERFORMANCE (CHECK IF ACCEPTABLE) Procedural Evaluation Student's Name Instructor's Signature Comments: Grade Date TABLE 1A WAVELENGTH (nm) ABSORBANCE TABLE 1B WAVELENGTH ABSORBANCE 550 0.477 512 0.769 500 0.762 511 0.773 450 0.355 400 0.134 510 0.780 509 0.787 WAVELENGTH (nm) ABSORBANCE 508 0.781 520 0.748 507 0.764 515 0.759 510 0.780 505 0.771 500 0.771 495 0.651 490 0.590
Transcribed Image Text:SHEET 7-1 SEQUENCE 10b 11 12 PROCEDURAL STEP NOTE: Sample color provides a good indication of what wavelength region to use. A yellow solution absorbs light in the 400- to 500-nm region. A red solution absorbs light between 500 and 600 nm, and blue solutions absorb light in the 600- to 700-nm range. A nanometer (nm) equals 10-8 meter. When actually doing an assay with high and low concentration, check to be sure there is enough difference in absorbance between low and high analyte concentrations by measuring other standard solutions with expected low and high concentrations. Remove the final cuvette from the spectrophotometer, carefully rinse the cuvettes with distilled water. Store spectrophotometer cuvettes, keeping them separate from regular test tubes. If the instrument is stored on the benchtop, cover with a dust cover. Otherwise, unplug and place on a storage shelf. INSTRUCTOR- OBSERVED ACCEPTABLE PERFORMANCE (CHECK IF ACCEPTABLE) Procedural Evaluation Student's Name Instructor's Signature Comments: Grade Date TABLE 1A WAVELENGTH (nm) ABSORBANCE TABLE 1B WAVELENGTH ABSORBANCE 550 0.477 512 0.769 500 0.762 511 0.773 450 0.355 400 0.134 510 0.780 509 0.787 WAVELENGTH (nm) ABSORBANCE 508 0.781 520 0.748 507 0.764 515 0.759 510 0.780 505 0.771 500 0.771 495 0.651 490 0.590
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