Experiment 1: Met #18 (M, single-letter code) was replaced by either Q, G, P, L, C, S, or A. In the binding assay, all of the variants and the original M13-EPI were mixed together in equal amounts and then applied to the thrombin-bound beads. Then, the percentage of each variant eluted by the low pH buffer was determined. Experiment 2: The assay is similar to that described in Experiment 1, but in this case, the amino acid Glu #31 (E) was replaced by either Q, G, P, L, C, S, or A. Experiment 3: The assay is similar to that described in Experiment 1, but in this case, the amino acid Ile #34 (I) was replaced by either Q, G, P, L, C, S, or A. a) Based on the results in the table below, briefly explain the significance of each experiment as it relates to the specificity of inhibition and the percentage of each variant found in the low pH eluate. In the table, the variants are identified by the amino acid found at positions #18, #31, and #34 in Experiments 1, 2, and 3, respectively. (9 pts) Experiment 1, AA at #18 Experiment 2, AA at #31 Experiment 3, AA at #34 Predominant M13-EPI variant(s) in eluate G E I, Q, G, P, L, C, S, A Minor M13-EPI variant(s) in eluate Q, P, L, C, S, A, M Q, G, P, L, C, S, A None

Experiment 1: Met #18 (M, single-letter code) was replaced by either Q, G, P, L, C, S, or A. In the binding assay, all of the variants and the original M13-EPI were mixed together in equal amounts and then applied to the thrombin-bound beads. Then, the percentage of each variant eluted by the low pH buffer was determined. Experiment 2: The assay is similar to that described in Experiment 1, but in this case, the amino acid Glu #31 (E) was replaced by either Q, G, P, L, C, S, or A. Experiment 3: The assay is similar to that described in Experiment 1, but in this case, the amino acid Ile #34 (I) was replaced by either Q, G, P, L, C, S, or A. a) Based on the results in the table below, briefly explain the significance of each experiment as it relates to the specificity of inhibition and the percentage of each variant found in the low pH eluate. In the table, the variants are identified by the amino acid found at positions #18, #31, and #34 in Experiments 1, 2, and 3, respectively. (9 pts) Experiment 1, AA at #18 Experiment 2, AA at #31 Experiment 3, AA at #34 Predominant M13-EPI variant(s) in eluate G E I, Q, G, P, L, C, S, A Minor M13-EPI variant(s) in eluate Q, P, L, C, S, A, M Q, G, P, L, C, S, A None

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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