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Basic Clinical Laboratory Techniques 6E

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter2: Basic Hematology

Section2.3: Microhematocrit

Problem 3RQ

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6. DNA electrophoresis uses polyacrylamide gel for separation.
a) True
b) False
7. Agarose is a natural protein made from agar.
alTrue
b)False
8. Agarose gel electrophoresis works well for DNA because of its small pores
compared SDS-PAGE Electrophoresis.
a)True
b)False
9. DNA can be visualized due by staining using Bradford reagent.
a)True
b)False
10. Unlike proteins, nucleic acids can have both negative and positive charges.
a)True
b)False

AGAROSE GEL ELECTROPHORESIS PRELAB
ANSWER ALL QUESTIONS
1. Type of nucleotide is one of the factors that influence electrophoretic mobility.
a) True
b) False
2. Electrophoresis is used for DNA separation and not proteins.
a) True
b) False
3. DNA Polymerase is an enzyme to cut DNA into fragments for electrophoresis.
a)True
b)False
4. Electrophoresis apparatus consists of Gel buffer, chamber and DNA separator.
a) True
b) False
5. During electrophoresis, DNA fragments collect at the Cathode.
a) True
b) False

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You followed all the steps correctly with a kit you bought last week. However, NO purified DNA came out of the miniprep (More than one answer possible). a) The plasmid was lost during culture growth b) Your tears from doing the same experiment again and again contaminated the reagents, causing them not to work c) Depurination of the DNA led to loss of genetic material d) Incomplete lysis of the cells after adding lysis buffer
Solution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual). Which effect does NaOH have on E. coli DNA A) It denatures genomic DNA and plasmid DNA. B) It denatures genomic DNA and but not plasmid DNA. C) It denatures plasmid DNA but not genomic DNA. D) It denatures neither genomic DNA nor plasmid DNA. E) This is unpredictable.
Solution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual). Which effect does NaOH have on E. coli DNA A) It denatures genomic DNA and plasmid DNA. B) It denatures genomic DNA and but not plasmid DNA. C) It denatures plasmid DNA but not genomic DNA. D) It denatures neither genomic DNA nor plasmid DNA. E) This is unpredictable. Which statement on the migration of DNA fragments through agarose gels is false A) Small fragments migrate faster than larger fragments because they can move faster through the agarose pores. B) Large fragments migrate faster than small fragments because they carry more negative charges. C) DNA fragments migrate towards the positive pole. D) Supercoiled DNA may migrate significantly different through the gel than linear DNA of equal size. E) The higher the agarose concentration the better the separation of smaller fragments as compared to larger fragments.
Dear All, Question for review 1. Discuss the following terminologies a) Mesosomes b) Plasmids c) LPS d) O antigen e) H antigen f) Capsule g) flagella h) pili i) endospore 2. Basic properties of nucleoid 3. The structure and chemical components of bacterial cell walls
question 2. To detect trisomy 18 in amniotic fluid cells the most optimal probe type to use is A) M-BCR/ABL probe B) DiGeorge Probe C) Whole chromosome paint probe D) A probe specific for highly repeated alphoid DNA sequences
How do we separate plasmid and chromosomal DNA during the alkaline plasmid screen?
At what stage of the culture should bacterial colonies be harvested for plasmid DNA extraction? How about for genomic DNA extraction? What distinguishes the xanthogenate-based methodology from the traditional phenol/chloroform method for isolating DNA from bacteria? What makes potassium ethyl xanthogenate efficient in isolating DNA from a variety of microorganisms?
12th, 11:59 PM Selectable markers allow Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a) a plasmid to replicate b) you to tell if the plasmid is present in a cell c) you to tell if the you have inserted a piece of DNA into a plasmid d) none of the above a. 1o C. dl
Which of the following is true of the gel electrophoresis shown? A) the power source has not been turned on yet. B) the three wells contained the same DNA molecules. C) well #1 had fewer molecules of DNA than well #2 or #3 D) well #3 had more different sized molecules of DNA than well #1 or #2 Which of the following statements is TRUE about the band of molecules labeled X ( close to where it says positive electrode). A) it contains DNA fragments that are shorter than the ones in any of the other bands. B) it contains fewer DNA molecules than any of the other bands from lane 3. C) it contains more negatively charged particles than any other band on the gel. D) it contains fewer genes than any of the other bands on the gel.
tudent forgot to add GelRed in to the gel mixture when he prepared the Agarose Gel. What will be the problem caused by his mistake? A) He will not be able to securely deposit his samples in the wells, the samples will disperse into the buffer B) The student will have a hard time visualizing how far his samples have traveled in the gel to disconnect the power supply in time C) The student will not be able to visualize the DNA under the UV light D) The student 's DNA will get degraded during the process of electrophoresis E) More than one of the answer choices are correct
Why is it important to use a hyperthermophilic DNA polymerase in PCR? a) Because only hyperthermophiles have DNA polymerases. b) Because hyperthermophilic DNA polymerase is able to resist the saline reaction conditions. c) Because hyperthermophilic DNA polymerase is faster than other polymerases. d) Because hyperthermophilic DNA polymerase is able to resist denaturation at 95℃.
Pipetting 1. Explain why a micropipettor is an important instrument in biochemistry labs. 2. Describe the basic components (and function) of a micropipettor. Bacterial Techniques 1. Define the following. (a) Serial Dilution (b) Streak Plating (c) Spread Plating Transformation 1. Define bacterial transformation and explain why it is an important method in biochemistry labs. 2. Describe (figure, narrative) a plasmid and describe the basic components of a plasmid. 3. What role does CaCl2 play in bacterial transformation? 4. What role does heat shock play in bacterial transformation? Plasmid Isolation 1. Describe the roles the following play in plasmid purification. (a) Lysis Buffer (b) Neutralization Buffer (c) Elution Buffer

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