6. DNA electrophoresis uses polyacrylamide gel for separation.a) Trueb) False7. Agarose is a natural protein made from agar.alTrueb)False8. Agarose gel electrophoresis works well for DNA because of its small porescompared SDS-PAGE Electrophoresis.a)Trueb)False9. DNA can be visualized due by staining using Bradford reagent.a)Trueb)False10. Unlike proteins, nucleic acids can have both negative and positive charges.a)Trueb)False AGAROSE GEL ELECTROPHORESIS PRELABANSWER ALL QUESTIONS1. Type of nucleotide is one of the factors that influence electrophoretic mobility.a) Trueb) False2. Electrophoresis is used for DNA separation and not proteins.a) Trueb) False3. DNA Polymerase is an enzyme to cut DNA into fragments for electrophoresis.a)Trueb)False4. Electrophoresis apparatus consists of Gel buffer, chamber and DNA separator.a) Trueb) False5. During electrophoresis, DNA fragments collect at the Cathode.a) Trueb) False

DNA is composed of nucleotides attached via phosphodiester bonds. DNA act as genetic material in…

Answered: 6. DNA electrophoresis uses…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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All of the following are performed during restriction fragment length polymorphism analysis. 1. splitting of double-stranded into single-stranded DNA 2. gel electrophoresis 3. autoradiography 4. immersion in radioactive probes 5. digestion of DNA with restriction endonucleases 6. use of a positive charge to transfer single-stranded DNA from a gel to a membrane. The correct sequence of these operations is what
What is the relationship between fluorescence intensity of a spot and the amount of DNA in a sample for a Virochip DNA microassay? From the following which is the best choice Less DNA results in greater fluorescene since the laser passes through he sample eaiser More DNA equals to more intense fluorescence since the DNA makes a layer to refelct the laser light More DNA equals to more intense fluorescence since of more dye on the DNA is fluorescene More DNA results in to more fluorescence since there's more DNA to be excted by the laser None of the above
Q11) Explain, using the pCR 2.1 vector, what we would see if we try to grow cells on a plate with ampicillin and Xgal in each of these scenarios:a) transformation not successful (meaning the plasmid did not go inside the bacteria)b) transformation was successful, but no insert in LacZ gene (plasmid went in the bacteria but there was no inserted DNA in the LacZ gene of the plasmid)c) transformation and the ligation reactions were successful (got the plasmid in, and the plasmid is carrying extra DNA in the LacZ region, yay)
please answer the following question
There are 6 parts to this question: This is a follow up to the prior question regarding the replication of the DNA strand below. The DNA strand is here for your reference and you do not need to do anything with or to it. TC GATATCGG AGCTATAGCC c) what enzyme separated the parental DNA template strands, d) what bonds were broken? e) what enzyme replicates DNA f) before DNA can be replicated/copied, what must be laid down to allow the enzyme in "e" to replicated the DNA (be specific)? g) our DNA is replicated in many "pieces", what enzyme connects these many "pieces" into one continuous DNA strand that becomes the sister chromatid? h) during what specific phase of the cell cycle does this DNA replication process occur? (This should be a review question from last topics we covered).
Based on the quantitation data, please indicate how you would dilute the following samples. Fill in each blank with the appropriate information. Item 1: DNA concentration - 22.6 ng/uL Dilution factor - 1: Add uL DNA extract to uL buffer Final dilution concentration = ng/ul Item 4: DNA concentration - 3.75 ng/uL Dilution factor - 1: Add uL DNA extract to uL buffer Final dilution concentration = ng/ul

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6. DNA electrophoresis uses polyacrylamide gel for separation. a) True b) False 7. Agarose is a natural protein made from agar. a)True b)False 8. Agarose gel electrophoresis works well for DNA because of its small pores compared SDS-PAGE Electrophoresis. a)True