All of the following are needed for PCR except: A) dNTP nucleotide monomers B) Oligonucleotide primers C) Restriction endonuclease D) Template DNA E) DNA polymerase

Q: In pcr experiment, Does electrophoresis show that only DNA products of the desired size are present?…

A: A DNA sequence may be amplified trillions of times via PCR, yielding enough DNA copies for further…

Q: 24) DNA synthesis and sequencing 25) Restriction enzymes 26) Techniques to determine gene function

A: DNA synthesis and sequencing techniques enable the study of genetic information, while restriction…

Q: Why is it important to use a hyperthermophilic DNA polymerase in PCR? a) Because only…

A: PCR polymerase chain reaction is a biotechnology technique used for amplification of DNA.

Q: ou followed all the steps correctly with a kit you bought last week. However, NO purified DNA came…

A: Plasmids exist naturally in bacterial cells . In some cases bacteria need to pick up plasmid from…

Q: Solution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual).…

A: INTRODUCTION E. coli E. coli  are small gram negative motile bacilli which mainly cause urinary…

Q: Primer design for SLIC cloning and the protocol

A: Hi! Thank you for the questions. As you have posted multiple questions, I will be answering the…

Q: 33 - The correct procedures for gene cloning are given as a) PCR/transformation/ligation/restriction…

A: Gene cloning is the method of production of numerous copies of a gene fragment.

Q: Lane 5 in the gel above displays the pBashi cleavage products after restriction digest with the…

A: The type of restriction enzymes that cut double stranded DNA at a particular spot is known as…

Q: There are four possible observations that suggest an error occurred in a PCR reaction: a) Sequence…

A: PCR reaction is a method used to amplify a small sample into millions of small ones which help the…

Q: Recombinant DNA construction involvesa) Cleaving DNA with restriction endonuclease and joining with…

A: Genetic engineering is the branch of biological science that can input alteration of DNA in an…

Q: Why is it possible to visualize a PCR product on an agarose gel even if the template genome is…

A: Gel electrophoresis is a technique used in the separation of macromolecules such as proteins and DNA…

Q: What are the reasons why there needs to be more than 10 cycles in the PCR process? Name 3 reasons.

A: DNA sequences can be amplified in vitro using the PCR (Polymerase Chain Reaction) method. A…

Q: What are the advantages of using a recombinant DNA product from a human source instead of a product…

A: GENETIC ENGINEERING:- It is a modification in genes that is done using biotechnology ( Recombinant…

Q: Plasmids are the only vectors currently available for use in recombinant procedures.True or false?

A: The technology used for joining different DNA molecules from different species is known as…

Q: To make the recombinant plasmid permeable to DNA molecules, which of the chemicals is added?a)…

A: The correct option is  CaCl2 (b) CaCl2 is the chemical which is added to prevent degradation of the…

Q: What are the disadvantages of doing DNA sequencing?

A: DNA sequencing is a process of identifying the order of nucleic acid bases i.e. adenine, guanine,…

Q: E.cor1 is aa) DNA ligase enzymeb) Restriction endonucleasec) A vector used for insulin synthesisd) A…

A: Bacteria are member of large group of unicellular microorganisms. Bacteria have cell walls, but lack…

Q: Which of the following is helpful in distinguishing DNA of one individual from another?a) PCRb)…

A: DNA is found in almost every living cell. However, whether that cell possesses a special…

Q: Why is it important to include a selective marker gene on a plasmid? O a) So that you can select…

A: Bacteria are common source of plasmids. For the sole purpose of Cloning experiments, new plasmids…

Q: How are "sticky ends" generated in DNA? A) by gel electrophoresis O B) by cutting with restriction…

A: Sticky ends or the conhesive ends are the overhangs created in a Dna strand, generally using an…

Q: Use the figure of a plasmid below to answer the following questions. 43) Which enzyme was used to…

A: Bacteria contain Chromosomal DNA as a genetic material but apart from that it also possess rounded…

Q: y is DNA ligase so important in recombinant DNA technology? a.) It causes DNA to make multiple…

A: Recombination is the process of breaking and recombining fragments of DNA to create new allele…

Q: What is Recombinant DNA technology ? Who created this ?

A: DNA is the genetic material in all the organisms except certain viruses. It is a polymer of…

Q: Polymerase Chain Reaction (PCR) works by exposing the reaction to a series of temperature changes.…

A: Polymerase chain reaction (PCR) is a scientific method that exponentially multiplies a single copy…

Q: 16. Which of the following enzyme repairs the DNA backbone during molecular cloning (that is you are…

A: Answer: Introduction: Molecular cloning is a genetic methods in biotechnology, which are utilized to…

Q: Based on the following image: A) Identify the largest DNA fragment in sample 5. Explain your…

A: In the given question, an agarose gel is shown which is having bands of DNA in different lanes. DNA…

Q: For what purpose is DNA fingerprinting used A) to sequence DNA from bacteria B) to separate DNA…

A: DNA fingerprinting:                 A method of isolating and identifying variable elements within…

Q: 2a) With the aid of diagrams, describe the critical role of restriction enzymes in genetic…

A: Genetic Engineering is combining DNA from two or more different organisms to create recombinant DNA.

Q: 8) Capillary electrophoresis involves a) Fragmentation of the DNA strands b) High resolution…

A: Capillary electrophoresis is an experimental methodology that provides an applied voltage to…

Q: )Which of the following is not an application of PCR? a) Determine if two people are related. b)…

A: PCR(polymerase chain reaction) is a quantitative technique responsible for the amplification of…

Q: The percentage of the agarose gel is important because a) it influences the rate of migration of…

A: 1. Agarose gel is widely used for the separation of DNA and RNA molecules and their visualization.…

Q: PCR can be used __________. a) to increase the number of specific DNA fragments b) to modify the…

A: PCR or Polymerase Chain Reaction is a method of molecular biology which is used to amplify a DNA…

Q: g best describes the complete sequence of steps occurring during every cycle of PCR? I-The primers…

A: A laboratory method used to make many copies of a specific piece of DNA from a sample that contains…

Q: What is a reason that a cut digest insert would not match up in length with a PCR insert in gel…

A: Electrophoresis describes the separation of charged particles and their migration in the influence…

Q: Which of the following enzyme is used in PCR? a) EcoRII b) EcoRI c) Tag DNA polymerase d) HRP

A: PCR (Polymerase Chain Reaction) is a widely used technique in molecular biology that amplifies a…

Q: High Frequency Recombination results in which of the following? O 1) Plasmid DNA incorporated into…

A: The bacterial DNA is arranged as a circular chromosome. The bacterial genome has a single origin of…

Genetic Recombination

Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.

Microbial Genetics

Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.